Regulation of cold-responsive Cor/Lea genes and their transcription factors by the major freezing tolerance locus Fr-1 in wheat
Regulation of cold-responsive Cor/Lea genes and their transcription factors by the major freezing tolerance locus Fr-1 in wheat
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小麦抗冻主位点 Fr-1 对冷响应 Cor/Lea 基因及其转录因子的调控
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
S. Pandalai
中科院分区：
文献类型：
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作者：
F. Kobayashi;S. Takumi;C. Nakamura;S. Pandalai
VRN-1和FR-1是众所周知的主要定量特质基因座，分别确定了普通小麦的开花和冬季野性的春化。这两个基因座连接在同型组5染色体的长臂上。最近的研究揭示了CRT（C-Repeat）/DRE（脱水响应元件）的存在。在小麦的许多COR（冷响应）/LEA（晚期 - 胚胎生成）基因的5'上游区域中，Abre（脱甲酸（ABA）响应元件）和其他冷响应基序。我们已经确定了WCBF2（小麦CRT结合因子2）基因，该基因编码识别CRT基序的推定转录因子，其转录水平在低温应激下的COR/LEA基因表达之前迅速增加。 WCBF2级联反应是与ABA无关的，并且与冷适应密切相关，而冷响应性VRN-1候选基因WAP1的表达显然与WCBF2基因的表达无关。其他冷响应转录因子基因WDREB2和WLIP19的表达弱控制，而与ABA依赖性途径有关的WAB15转录因子基因似乎独立于FR-1。我们的结果表明，通过普通小麦中的CBF/DREB（DRE结合）途径，VRN-1和FR-1基因座的基因型之间的低温应力反应有自适应关系。
Vrn-1 and Fr-1 are well known major quantitative trait loci respectively determining vernalization for flowering and winter hardiness in common wheat. The two loci are linked on the long-arms of homoelogous group 5 chromosomes. Recent studies have revealed the presence of CRT (C-repeat)/DRE (dehydration-responsive element). ABRE (abscisic acid (ABA)-responsive element) and other cold-responsive motifs in the 5' upstream regions of many Cor (cold-responsive)/Lea (late-embryogenesis-abundant) genes in wheat. We have identified a WCBF2 (wheat CRT-binding factor 2) gene that encodes a putative transcription factor recognizing the CRT motif and whose transcript level rapidly increases prior to the Cor/Lea gene expression under low temperature stress. The WCBF2 cascade is ABA-independent and tightly associated with cold acclimation, whereas expression of the cold-responsive Vrn-1 candidate gene WAP1 is apparently not correlated with expression of the WCBF2 gene. Expression of other cold-responsive transcription factor genes, WDREB2 and WLIP19, are weakly controlled by Fr-1, while WAB15 transcription factor gene involved in the ABA-dependent pathway appears to act independently of Fr-1. Our results suggest an adaptive relationship on the low temperature stress response between the genotypes of the Vrn-1 and Fr-1 loci through the CBF/DREB (DRE-binding) pathway in common wheat.