A SIMPLE METHOD FOR PREMATURE CHROMOSOME CONDENSATION INDUCTION IN PRIMARY HUMAN AND RODENT CELLS USING POLYETHYLENE-GLYCOL
A SIMPLE METHOD FOR PREMATURE CHROMOSOME CONDENSATION INDUCTION IN PRIMARY HUMAN AND RODENT CELLS USING POLYETHYLENE-GLYCOL
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DOI：
10.1007/bf01574257
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
MAILLIE, HD
中科院分区：
文献类型：
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作者：
PANTELIAS, GE;MAILLIE, HD
尽管聚乙二醇（PEG）是一种有效的融合剂，但它并没有被广泛利用作为仙台病毒的替代品，用于诱导过早的染色体凝聚（PCC）。一个简单的，快速的和可重复的PEG协议的原代细胞悬浮液中，它允许令人满意的细胞融合和PCC指数，在同一时间高细胞活力和低巨，多核细胞的形成。提供了在人和大鼠淋巴细胞、啮齿动物脾细胞以及新生小鼠的脾和全身细胞中PEG介导的融合和过早染色体凝聚诱导的技术细节。提出了该方法的进一步应用。
Even though polyethylene glycol (PEG) is a potent fusogen, it was not widely exploited as an alternative to the Sendai virus for premature chromosome condensation (PCC) induction. A simple, rapid and reproducible PEG protocol for primary cells in suspension is presented which allows satisfactory cell fusion and PCC indices, giving at the same time high cell viability and low giant, multinucleated cell formation. Technical details for PEG-mediated fusion and premature chromosome condensation induction in human and rat lymphocytes, rodent spleen cells, and spleen and whole body cells of newborn mice are provided. Further applications of the method are suggested.