The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers
The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers
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DOI：
10.1016/j.jphotobiol.2011.10.001
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发表时间：
2012-01-05
影响因子：
5.4
通讯作者：
Rodrigues, Lidiany K. A.
中科院分区：
文献类型：
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作者：
Rolim, Juliana P. M. L.;de-Melo, Mary A. S.;Rodrigues, Lidiany K. A.
几种光敏剂在没有标准化的情况下被用于对抗口腔细菌。单线态氧(O-1(2))是一种侵略性化学物质，可通过细胞凋亡或坏死来杀死细胞。目的：比较相同浓度不同光敏剂的光动力疗法(PDT)对变形链球菌的抗菌活性。此外，还测定了各光敏剂的O-1(2)生成量。用发光二极管激活光敏剂亚甲基蓝(MB)、邻甲苯胺蓝(TbO)和孔雀石绿(MG)(163.5 mM)。波长为636 nm)，而曙红(EOS)、红素(ERI)和孟加拉玫瑰(RB)则用波长为570 nm的固化光照射。光源工作在24J cm(-2)。对于每种光敏剂，在以下实验条件之一下进行40次随机分析(n=10)：无光照射或光敏剂、仅照射、仅光敏剂或在光敏剂存在的情况下照射。治疗后，将系列稀释度的变形链球菌接种到脑心输注琼脂上，以每毫升克隆形成单位(CFU mL(-1))测定活性。色氨酸光氧化分析了O-1(2)的产生，并估算了其衰减常数。结果采用单因素方差分析和Tukey-Kramer检验(p<；0.05)。与各自未处理的对照相比，在光敏剂TBO和MG存在的情况下，PDT有效地将变形链球菌的数量分别减少了3和1.4个对数(p<；0.01)。MB产生的O-1(2)是TBO的1.3倍，两者产生的单线态氧浓度都明显高于其他光敏剂。由于在体外大量产生O-1(2)并不表明O-1(2)是在细菌的活性部位产生的，所以对变形链球菌的杀菌作用与体外单线态O-2的生成率无关。在体外变形链球菌实验表明，TBO是唯一的光敏剂，有效地减少了99.9%的这些微生物。(C)2011爱思唯尔B.V.保留所有权利。
Several photosensitizers have been used against oral bacteria without standardization. Singlet oxygen (O-1(2)) is an aggressive chemical species that can kill cells through apoptosis or necrosis. Objective: to compare the antimicrobial activity of photodynamic therapy (PDT) with different photosensitizers at the same concentration against Streptococcus mutans. In addition, the O-1(2) production of each photosensitizer was determined. The photosensitizers (163.5 mu M) methylene blue (MB), toluidine blue ortho (TBO) and malachite green (MG) were activated with a light-emitting diode (LED;). lambda = 636 nm), while eosin (EOS), erythrosine (ERI) and rose bengal (RB) were irradiated with a curing light (lambda = 570 nm). Light sources were operated at 24J cm(-2). For each photosensitizer, 40 randomized assays (n = 10 per condition) were performed under one of the following experimental conditions: no light irradiation or photosensitizer, irradiation only, photosensitizer only or irradiation in the presence of a photosensitizer. After treatment, serial dilutions of S. mutans were seeded onto brain heart infusion agar to determine viability in colony-forming units per milliliter (CFU mL(-1)). Generation of O-1(2) was analyzed by tryptophan photooxidation, and the decay constant was estimated. Results were analyzed by one-way ANOVA and the Tukey-Kramer test (p < 0.05). PDT with irradiation in the presence of the photosensitizers TBO and MG was effective in reducing S. mutans counts by 3 and 1.4 logs, respectively (p < 0.01), compared to their respective untreated controls. MB generated 1.3 times more O-1(2) than TBO, and both produced significantly higher concentrations of singlet oxygen than the other photosensitizers. Since in vitro bulk O-1(2) production does not indicate that O-1(2) was generated in the bacterial activity site, the bactericidal action against S. mutans cannot be related to in vitro singlet O-2 generation rate. in vitro S. mutans-experiments demonstrated TBO as the only photosensitizer that effectively reduced 99.9% of these microorganisms. (C) 2011 Elsevier B.V. All rights reserved.