CXXC5 regulates differentiation of C2C12 myoblasts into myocytes
CXXC5 regulates differentiation of C2C12 myoblasts into myocytes
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CXXC5 调节 C2C12 成肌细胞分化为肌细胞
DOI：
10.1007/s10974-014-9400-2
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发表时间：
2014-12-01
影响因子：
2.7
通讯作者：
Wang, Yuequn
中科院分区：
文献类型：
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作者：
Li, Guangming;Ye, Xiangli;Wang, Yuequn
CXXC5是CXXC型锌指结构域蛋白家族的一员，被认为在基因转录、细胞黏附和细胞骨架组织中发挥作用。以往的研究表明，CXXC5在骨骼肌中有表达，但它是否调控骨骼肌发生尚不清楚。在这里，我们利用荧光素酶基因记者筛选了CXXC5可能参与的信号通路。结果表明，CXXC5显著提高了骨骼肌分化相关基因启动子的活性。因此，我们研究了C2C12成肌细胞中CXXC5在骨骼肌发生过程中的作用。我们的研究结果表明，C2C12成肌细胞中CXXC5的过表达促进了肌细胞的分化，而CXXC5的RNAi干扰显著抑制了C2C12成肌细胞的分化。本研究提示CXXC5在骨骼肌发生过程中起着重要的调控作用。
CXXC5 is a member of the CXXC-type zinc-finger domain containing protein family, which is suggested to function in gene transcription, cell adhesion and cytoskeleton organization. Previous studies have revealed that CXXC5 is expressed in skeletal muscle, but whether it regulates skeletal myogenesis is yet unknown. Here, we screened for the possible signaling pathways in which CXXC5 might participate using luciferase gene reporters. The results indicated that CXXC5 significantly increased the activities of the promoters of genes involved in skeletal muscle differentiation. We therefore studied the role of CXXC5 during skeletal myogenesis in C2C12 myoblasts. Our findings suggest that overexpression of CXXC5 in C2C12 myoblasts facilitated myocyte differentiation, while RNAi interference of CXXC5 significantly inhibited the differentiation of C2C12 myoblasts. This study suggests that CXXC5 plays a significant role in regulating skeletal myogenesis.