Transcriptional Profiling Reveals a Time-of-Day-Specific Role of REVEILLE 4/8 in Regulating the First Wave of Heat Shock-Induced Gene Expression in Arabidopsis
Transcriptional Profiling Reveals a Time-of-Day-Specific Role of REVEILLE 4/8 in Regulating the First Wave of Heat Shock-Induced Gene Expression in Arabidopsis
复制标题
转录分析揭示了 REVEILLE 4/8 在调节拟南芥第一波热休克诱导基因表达中的特定时间作用
DOI：
10.1105/tpc.19.00519
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发表时间：
2019-10-01
期刊：
影响因子：
11.6
通讯作者：
Tang, Wenqiang
中科院分区：
文献类型：
--
作者：
Li, Bingjie;Gao, Zhihua;Tang, Wenqiang
虽然对植物对热激的反应已经有了很多了解，但植物如何感受高温以及导致热激调控基因表达的主要热激信号转导途径仍然知之甚少。为了鉴定介导HS调节的基因表达的主要转录因子及其靶基因，进行RNA测序以检测拟南芥（Arabidopsis thaliana）中其表达被HS快速改变的基因。结果显示，HS处理仅5分钟后就诱导了几个基因，表明HS信号传导发生得非常迅速。HS处理10分钟上调的基因启动子中的顺式元件的分析确定了热休克因子A1（HSFA 1）和昼夜节律钟蛋白REVEILLE 4（RVE 4）和RVE 8作为独立介导早期HS诱导的基因表达的必需转录因子。利用hsfa 1a/B/d/e和rve 4/8突变体，我们鉴定了HSFA 1 s或RVE 4/8依赖的早期HS诱导基因的子集，并显示RVE 4/8部分地通过调节下游转录因子乙烯应答因子53（ERF 53）和ERF 54的表达来调节植物耐热性，特别是在中午前后。这些发现揭示了一个潜在的转录调控层次管理HS诱导的基因表达的第一波。他们还提供了重要的洞察机制，昼夜节律钟门耐热性和准备植物暴露于高温在白天。
Although much is known about plant responses to heat shock (HS), how plants sense high temperature and the primary HS signal transduction pathway leading to HS-regulated gene expression are still poorly understood. To identify primary transcription factors that mediate HS-regulated gene expression and their target genes, RNA sequencing was performed to detect genes whose expression is rapidly altered by HS in Arabidopsis (Arabidopsis thaliana). The results showed several genes were induced after only 5 min of HS treatment, suggesting that HS signaling occurs very rapidly. Analysis of the cis-elements in the promoters of genes upregulated by 10 min of HS treatment identified HEAT SHOCK FACTOR A1s (HSFA1s) and circadian clock proteins REVEILLE4 (RVE4) and RVE8 as essential transcription factors that independently mediate early HS-induced gene expression. Using hsfa1a/b/d/e and rve4/8 mutants, we identified subsets of HSFA1s- or RVE4/8-dependent early HS-induced genes and showed RVE4/8 regulate plant thermotolerance partially by regulating the expression of downstream transcription factors ETHYLENE RESPONSIVE FACTOR53 (ERF53) and ERF54, specifically around noon. These findings reveal a potential transcriptional regulatory hierarchy governing the first wave of HS-induced gene expression. They also provided important insight into the mechanism by which the circadian clock gates thermotolerance and prepares plants for exposure to high temperatures during the day.