Appearance of myelin proteins in rat sciatic nerve during development
Appearance of myelin proteins in rat sciatic nerve during development
复制标题
大鼠坐骨神经发育过程中髓磷脂蛋白的出现
DOI：
10.1016/0006-8993(75)90137-7
复制
发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
P. Morell
中科院分区：
文献类型：
--
作者：
R. Wiggins;J. Benjamins;P. Morell
处死5至45日龄的大鼠，解剖其坐骨神经。通过在不连续蔗糖梯度上纯化的程序从这些坐骨神经制备髓鞘。在含有十二烷基硫酸钠的缓冲液中，通过不连续聚丙烯酰胺凝胶电泳检测不同年龄的整个坐骨神经的蛋白质和来自从这些坐骨神经分离的髓鞘的蛋白质。坐骨神经髓鞘中超过一半的蛋白质在凝胶上迁移为单一条带（P0）。在发育过程中，坐骨神经髓鞘的蛋白质分布只有微小的变化。与此相反，整个坐骨神经匀浆的聚丙烯酰胺凝胶模式显着变化，在5日龄和15日龄之间的发展。P0蛋白占坐骨神经总蛋白的比例从5日龄时的3%增加到15日龄时的13%，此后保持不变。其他几种蛋白质也是分离的髓鞘的特征，在这段时间内相对重要性增加。平行实验处理髓鞘形成的代谢参数，纳入腹膜内注射的[35S]硫酸硫脂，进行。硫苷脂的最大合成发生在6至16日龄之间，与坐骨神经中髓鞘蛋白的显著积累相一致。
Rats between 5 and 45 days of age were sacrificed and their sciatic nerves dissected. Myelin was prepared from these sciatic nerves by a procedure involving purification on discontinuous sucrose gradients. The proteins of whole sciatic nerves at different ages and the proteins derived from the myelin isolated from these sciatic nerves were examined by discontinuous polyacrylamide gel electrophoresis in buffers containing sodium dodecyl sulfate. Over half of the proteins of sciatic nerve myelin migrated in a single band on the gel (P0). There were only minor changes in the protein distribution of sciatic nerve myelin during development. In contrast, the polyacrylamide gel patterns of whole sciatic nerve homogenate changed markedly during development between 5 and 15 days of age. The amount of P0protein as a proportion of the total sciatic nerve protein increased from 3% at 5 days of age to 13% at 15 days of age after which it remained constant. Several other proteins which were also characteristic of the isolated myelin increased in relative importance during this time period. Parallel experiments dealing with a metabolic parameter of myelinogenesis, incorporation of intraperitoneally injected [35S]sulfate into sulfatide, were conducted. The maximum synthesis of sulfatide occurred between 6 and 16 days of age, coincident with the marked accumulation of myelin proteins in sciatic nerve.