FveRGA1, encoding a DELLA protein, negatively regulates runner production in Fragaria vesca
FveRGA1, encoding a DELLA protein, negatively regulates runner production in Fragaria vesca
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FveRGA1，编码 DELLA 蛋白，负向调节草莓的匍匐生长
DOI：
10.1007/s00425-017-2839-9
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发表时间：
2018-04-01
期刊：
影响因子：
4.3
通讯作者：
Zhang, Zhihong
中科院分区：
文献类型：
--
作者：
Li, Weijia;Zhang, Junxiang;Zhang, Zhihong
FveRGA1在嫩叶和茎尖等幼嫩组织中高度表达，并定位于细胞核。在林地草莓中，RNAi沉默FveRGA1产生了大量的跑者。因此，FveRGA1是控制草莓流道形成的关键基因。草莓的繁殖主要依靠滑穗，而控制滑穗产生的基因尚未得到很好的鉴定。外源施用最适浓度赤霉素（GA）可促进草莓栽培中流道的形成，并能加速DELLA蛋白的降解。为了研究DELLA蛋白是否参与了草莓叶片的产生，我们分析了草莓中所有的DELLA基因，并利用RT-PCR技术克隆了一个DELLA蛋白编码基因FveRGA1。亚细胞定位分析表明，FveRGA1定位于细胞核。转录分析表明，FveRGA1在草莓各器官中普遍表达，尤其是在嫩叶、叶柄和茎尖。采用RNA干扰（RNAi）技术，通过农杆菌介导的转化，研究了林地草莓‘Yellow Wonder’（YW）和‘Ruegen’（RG）中FveRGA1的功能。有趣的是，RNAi沉默的转基因植株在自然不奔跑的YW和RG株系中产生了许多奔跑，这表明FveRGA1是控制草莓奔跑形成的关键基因。本研究为揭示草莓流道形成的详细分子机制奠定了坚实的基础。
FveRGA1 was highly expressed in tender tissues such as young leaves and stem apices and was localized in the nucleus. RNAi silencing of FveRGA1 in non-runnering woodland strawberry produced many runners. FveRGA1 is thus a key gene controlling strawberry runner formation. The propagation of strawberry is mainly based on runners, while the genes controlling runner production have not been well characterized. Exogenous applications of optimum concentration gibberellins (GAs) promote runner formation in strawberry cultivation and GA can accelerate the degradation of DELLA proteins. To investigate whether DELLA proteins are responsible for runner production, we analyzed all the DELLA genes in Fragaria vesca and cloned a DELLA protein-encoding gene FveRGA1 in woodland strawberry using RT-PCR. Subcellular localization analysis indicated that FveRGA1 was localized in the nucleus. A transcription analysis suggested that FveRGA1 was expressed ubiquitously in all examined strawberry organs, especially in young leaves, petioles, and stem apices. RNA interference (RNAi) technology was carried out to investigate the function of FveRGA1 in woodland strawberry 'Yellow Wonder' (YW) and 'Ruegen' (RG) via an Agrobacterium-mediated transformation. Interestingly, the RNAi silencing transgenic plants in the naturally non-runnering YW and RG strains produced many runners, suggesting FveRGA1 as a key gene controlling strawberry runner formation. Our study lays a solid basis for unraveling the detailed molecular mechanism of runner formation in strawberry.