Evaluation of the ability of adhesives with antibacterial and remineralization functions to prevent secondary caries in vivo
Evaluation of the ability of adhesives with antibacterial and remineralization functions to prevent secondary caries in vivo
复制标题
具有抗菌和再矿化功能的粘合剂在体内预防继发龋能力的评价
DOI：
10.1007/s00784-021-04334-4
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发表时间：
--
影响因子：
3.4
通讯作者：
Li Jiyao
中科院分区：
文献类型：
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作者：
Liu Yifang;Yang Jiaojiao;Yang Yingming;Li Meng;Xu Hockin H.K.;Weir Michael D.;Zhou Xuedong;Liang Kunneng;Li Jiyao
③充填治疗的粘结界面是抗继发龋的薄弱环节。含有无定形磷酸钙（NACP）和甲基丙烯酸二甲氨基十六烷基酯（DMAHDM）的纳米颗粒的粘合剂已在体外被证明可以防止细菌产生酸并促进牙齿矿化。本研究旨在评价NACP和DMAHDM粘合剂预防继发龋的效果在vivo.Materials and MethodsArtificial cavity创建在大鼠模型上颌骨两侧的第一磨牙。一侧用含有NACP + DMAHDM的粘合剂处理，而另一侧用市售粘合剂作为对照。致龋喂养24天后，通过micro-CT和改良的Keyes评分法评估继发龋的程度。采用实时定量PCR（qPCR）和菌落形成单位（CFU）计数来评价材料的抗菌效果。结果在大鼠模型中，NACP + DMAHDM的粘合剂显示出良好的生物相容性，并有效地减少了细菌的数量。根据显微CT，实验组表现出极好的再矿化效果，与对照组的77.44 ± 7.22 vol% μ m相比，实验组的改良Keyes评分和34.16 ± 2.13 vol% μm的矿物质损失较低（P< 0.05），显示出极好的抑制继发龋的能力。临床意义含NACP和DMAHDM的粘接剂通过抑制细菌生长和促进牙釉质矿化，在临床牙科中具有很大的应用潜力。
ObjectiveThe bonding interface of dental filling therapy is the weak point in resisting secondary caries. Adhesives containing nanoparticles of amorphous calcium phosphate (NACP) and dimethylaminohexadecyl methacrylate (DMAHDM) have been demonstrated in vitro to prevent bacteria from producing acid and to promote tooth remineralization. The present study aimed to evaluate the efficacy of adhesive with NACP and DMAHDM to prevent secondary caries in vivo.Materials and methodsArtificial cavities were created on the first molar on both sides of the maxillary in a rat model. One side was treated with adhesive containing NACP + DMAHDM, while on the other side, a commercial adhesive served as control. After 24 days of cariogenic feeding, the degree of secondary caries was evaluated by micro-CT and a modified Keyes scoring method. Quantitative real-time PCR (qPCR) and colony-forming unit (CFU) counts were used to evaluate the antibacterial efficacy of the materials. Biocompatibility was also investigated.ResultsIn the rat model, the adhesive with NACP + DMAHDM showed excellent biocompatibility and effectively decreased the amount of bacteria. The experimental group demonstrated excellent remineralization effectiveness, with a lower modified Keyes score and mineral loss of 34.16 ± 2.13 vol% μm, compared with 77.44 ± 7.22 vol% μm in the control group, according to micro-CT (P< 0.05), showing excellent capacity to inhibit secondary caries.ConclusionsThe NACP-DMAHDM-containing adhesive exhibited good performance in preventing secondary caries in vivo.Clinical relevanceAdhesives containing NACP and DMAHDM have great potential for use in clinical dentistry to prevent secondary caries by inhibiting bacterial growth and promoting remineralization.