Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels
Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels
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DOI：
10.1182/blood-2007-06-094318
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发表时间：
2008-02-01
期刊：
影响因子：
20.3
通讯作者：
Jain, Rakesh K.
中科院分区：
文献类型：
--
作者：
Au, Patrick;Daheron, Laurence M.;Jain, Rakesh K.
组织工程需要形成一个全新的稳定的血管网络。由于其具有增殖、分化为内皮细胞和形成新血管的能力，血液来源的内皮祖细胞（EPCs）是用于工程血管的有吸引力的细胞来源。然而，内皮祖细胞衍生血管在体内植入的耐久性和功能尚不清楚。为此，我们在体内血管生成模型中直接比较了外周血和脐带血来源的EPCs生成的组织工程血管的形成和功能。我们发现成人外周血内皮祖细胞形成的血管不稳定，并在3周内消退。相比之下，脐带血内皮祖细胞形成功能正常的血管，持续时间超过4个月。这些血管表现出正常的血流，对大分子的选择性，以及在细胞因子激活的反应中诱导白细胞-内皮相互作用，类似于正常血管。因此，脐带血内皮祖细胞具有巨大的治疗潜力，应在血管工程中加以利用。
Tissue engineering requires formation of a de novo stable vascular network. Because of their ability to proliferate, differentiate into endothelial cells, and form new vessels, blood-derived endothelial progenitor cells (EPCs) are attractive source of cells for use in engineering blood vessels. However, the durability and function of EPC-derived vessels implanted in vivo are unclear. To this end, we directly compared formation and functions of tissue-engineered blood vessels generated by peripheral blood- and umbilical cord blood-derived EPCs in a model of in vivo vasculogenesis. We found that adult peripheral blood EPCs form blood vessels that are unstable and regress within 3 weeks. In contrast, umbilical cord blood EPCs form normal-functioning blood vessels that last for more than 4 months. These vessels exhibit normal blood flow, perm-selectivity to macromolecules, and induction of leukocyte-endothelial interactions in response to cytokine activation similar to normal vessels. Thus, umbilical cord blood EPCs hold great therapeutic potential, and their use should be pursued for vascular engineering.