Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure.
Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure.
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DOI：
10.1021/jp063996u
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发表时间：
2007-02
期刊：
影响因子：
--
通讯作者：
Jian Zhang;Yi Fu;J. Lakowicz
中科院分区：
文献类型：
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作者：
Jian Zhang;Yi Fu;J. Lakowicz
通过与2 0~6 0 0μ的M Ru(Bpy)(3)(2+)配合物合成了平均粒径为10 0 nm的标记二氧化硅小球。银壳层层沉积在微珠上，壳层厚度为5~50 nm。随着壳层厚度的增加，发射带变窄，强度增强。在高浓度下观察到了探针的自猝灭现象。用泊松统计方法分析了荧光团的自猝灭现象。估算的猝灭距离从6 nm延长到16 nm，壳层生长从0 nm增加到50 nm。此外，银壳上还标记了罗丹明6G。还观察到含有R6G的银-硅壳的荧光增强和寿命降低。我们发现，通过调节探针浓度和银壳厚度，Ru(Bpy)(3)(2+)标记的粒子可以比孤立的Ru(Bpy)(3)(2+)分子亮600倍。我们期望标记的金属核壳结构可以成为高灵敏度和/或单颗粒分析的有用探针。
Labeled silica beads with an average diameter of 100 nm were synthesized by incorporating with 20-600 μM Ru(bpy)(3) (2+) complexes. Silver shells were deposited on the beads layer-by-layer with the shell thickness of 5-50 nm. The emission band became narrower and the intensity was enhanced depending on the shell thickness. Self-quenching of the probe was observed at high concentration. Poisson statistics were employed to analyze self-quenching of the fluorophores. The estimated quenching distance was extended from 6 to 16 nm with shell growth from 0 to 50 nm. Moreover, the silver shells were also labeled with Rhodamine 6G. Fluorescence enhancement and reduced lifetime were also observed for silver-silica shell containing R6G. We found that by adjustment of probe concentration and silver shell thickness, a Ru(bpy)(3) (2+)-labeled particle could be 600 times brighter than an isolated Ru(bpy)(3) (2+) molecule. We expect labeled metal core-shell structures can become useful probes for high sensitivity and/or single particle assay.