Development of analytical method for bisphenol A in canned fish and meat by HPLC
Development of analytical method for bisphenol A in canned fish and meat by HPLC
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DOI：
10.3358/shokueishi.40.4_325
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发表时间：
1999-08-01
期刊：
影响因子：
--
通讯作者：
Wada, M
中科院分区：
文献类型：
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作者：
Takino, A;Tsuda, T;Wada, M
建立了一种分析方法。鱼和肉罐头中的双酚A(BPA)。样品用150mT的乙酸乙酯提取，有机层用150mT的5%氯化钠溶液清洗。萃取物经正己烷/乙腈脱脂后，用10%水脱水硅胶柱，用100mT的15%丙酮/正己烷洗脱，用50mT的60%丙酮/正己烷洗脱。洗脱液蒸发至干燥，然后溶解在甲醇中。色谱柱为Wakosil 5C18(4.6 mm i.d.×150 mm)，流动相为60%甲醇，检测波长为230 nm，流速为0.8 m L/min，20 g金枪鱼罐头牛排中添加1 mg双酚A的回收率为89.9+/-7.1(n=3)。用所建立的方法对市售的4种鱼罐头和3种肉类罐头进行了双酚A分析。双酚A的含量范围为319 ng/g，检出限为25 ng/g。该方法不使用氯仿和二氯甲烷等溶剂，对鱼肉罐头中的双酚A具有良好的检测效果。
An analytical method was developed. for bisphenol A (BPA) in canned fish and meat. A sample was extracted with 150 mt of ethyl acetate and the organic layer was washed with 150 mt of 5% NaCl solution. After defatting by n-hexane/acetonitrile partition, the extract was loaded on a 10% water-dehydrated silica gel column, washed with 100 mt of 15% acetone/ n-hexane and eluted with 50 mt of 60% acetone/n-hexane. The eluate was evaporated to dryness and dissolved in methanol. BPA was analyzed by HPLC [column, Wakosil 5C18 (4.6 mm i.d.X150 mm); mobile phase, 60% methanol; detection, 230 nm; flow rate, 0.8 mL/min].Recovery of BPA in 20 g of canned tuna steak (maguro steak) fortified with 1 mu g of BPA was 89.9+/-7.1 (n = 3). Four kinds of canned fish and three kinds of canned meat on the market were analyzed for BPA with the developed method. The ranges of BPA content were N.D.similar to 319 ng/g. The detection limit of BPA was 25 ng/g.This method was effective for BPA in canned fish and meat and does not use solvents such as chloroform and dichloromethane.