A practical guide to DNA extraction, PCR, and gene-based DNA sequencing in insects
A practical guide to DNA extraction, PCR, and gene-based DNA sequencing in insects
复制标题
昆虫 DNA 提取、PCR 和基于基因的 DNA 测序实用指南
DOI：
--
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
C. Moreau
中科院分区：
文献类型：
--
作者：
C. Moreau
分子生物学方法对于推断进化关系、实施多样化和地理范围进化分析以及推断感兴趣的性状的进化具有高度的信息性。虽然这些分子实验室方法在生命树中广泛使用，但在分类组中有特定的协议。由于这些原因，我提出的协议，我已经成功地用于DNA提取，聚合酶链反应（PCR），和循环测序的基因为基础的和有针对性的基因组测序的昆虫。这本实用指南并不详尽，我鼓励科学家根据他们感兴趣的分类组或特定的科学问题的需要使用，修改和分享这些详细的步骤。
Molecular methods have been highly informative for inferring evolutionary relationships, implementing diversification and biogeographic range evolution analyses, and inferring the evolution of traits of interest. Although these molecular laboratory methods are widely used across the tree of life, there are specific protocols that are useful within taxonomic groups. For these reasons I present protocols I have successfully used for DNA extraction, polymerase chain reaction (PCR), and cycle sequencing for gene-based and targeted genome sequencing of insects. This practical guide is not exhaustive and I encourage scientists to use, modify, and share these detailed steps as needed for their taxonomic group of interest or specific scientific question.