Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor.
Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor.
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DOI：
10.1210/mend-4-12-1956
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发表时间：
1990-12
影响因子：
--
通讯作者：
E. Spindel;E. Giladi;Paul Brehm;Richard H. Goodman;Thomas P. Segerson
中科院分区：
文献类型：
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作者：
E. Spindel;E. Giladi;Paul Brehm;Richard H. Goodman;Thomas P. Segerson
两栖动物四肽bombesin及其哺乳动物同源胃泌素释放肽是神经递质和旁分泌激素，对成纤维细胞和小细胞肺癌细胞系有丝分裂作用。利用电生理和荧光法分离鼠爪蟾卵母细胞表达的bombesin/胃泌素释放肽受体（BR）的cdna。微注射BR转录本的卵母细胞对浓度为1 × 10（-10）至1 × 10(-6) m的bombesin有反应，这些反应表现出同源脱敏，可以被bombesin拮抗剂特异性阻断。序列分析表明，该BR具有7个跨膜结构域和5个潜在的n -链糖基化位点。数据库分析表明，该BR与速激肽受体最同源。虽然酪氨酸激酶活性与BR功能相关，但在BR序列中没有酪氨酸激酶同源性。
The amphibian tetradecapeptide bombesin and its mammalian homolog gastrin-releasing peptide are neurotransmitters and paracrine hormones, and are mitogenic for fibroblast and small cell lung carcinoma cell lines. cDNAs encoding the bombesin/gastrin-releasing peptide receptor (BR) expressed by murine Swiss 3T3 fibroblasts were isolated using electrophysiological and luminometric Xenopus oocyte expression assays. Oocytes microinjected with BR transcripts responded to concentrations of bombesin from 1 x 10(-10) to 1 x 10(-6) M. These responses showed homologous desensitization and could be specifically blocked by bombesin antagonists. Sequence analysis showed that the BR has seven membrane-spanning domains and five potential N-linked glycosylation sites. Data base analysis showed that the BR is most homologous to the tachykinin receptors. Although tyrosine kinase activity has been associated with BR function, no tyrosine kinase homologies occur within the BR sequence.