Genetic requirements for cell division in a genomically minimal cell
Genetic requirements for cell division in a genomically minimal cell
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DOI：
10.1016/j.cell.2021.03.008
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发表时间：
2021-04-29
期刊：
影响因子：
64.5
通讯作者：
Strychalski, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Pelletier, James F.;Sun, Lijie;Strychalski, Elizabeth A.
基因组最小细胞（例如JCVI-Syn3.0）提供了一个平台来阐明核心生理过程的基因。尽管该最小细胞包含对种群生长必不可少的基因，但其单个细胞的生理仍然没有表征。为了研究JCVI-Syn3.0细胞中引人注目的形态变异，我们提出了一种表征细胞传播并确定影响细胞形态的基因的方法。微流体化学仪允许观察导致形态不规则的固有细胞动力学。 JCVI-Syn3.0中未保留的19个基因的基因组产生了JCVI-SYN3A，其形态与JCVI-Syn1.0相似。我们进一步鉴定了这19个基因中的7个，包括两个已知的细胞分裂基因，FTSZ和SEPF，一种未知底物的水解酶，以及编码未知功能的膜相关蛋白的四个基因，它们可以一起恢复与该表型相似的表型。 Jcvisyn1.0。该结果强调了基因组最小细胞中细胞分裂和形态的多基因性质。
Genomically minimal cells, such as JCVI-syn3.0, offer a platform to clarify genes underlying core physiological processes. Although this minimal cell includes genes essential for population growth, the physiology of its single cells remained uncharacterized. To investigate striking morphological variation in JCVI-syn3.0 cells, we present an approach to characterize cell propagation and determine genes affecting cell morphology. Microfluidic chemostats allowed observation of intrinsic cell dynamics that result in irregular morphologies. A genome with 19 genes not retained in JCVI-syn3.0 generated JCVI-syn3A, which presents morphology similar to that of JCVI-syn1.0. We further identified seven of these 19 genes, including two known cell division genes, ftsZ and sepF, a hydrolase of unknown substrate, and four genes that encode membrane-associated proteins of unknown function, which are required together to restore a phenotype similar to that of JCVIsyn1.0. This result emphasizes the polygenic nature of cell division and morphology in a genomically minimal cell.