Identification of tightly linked SSR markers for flower type in carnation (Dianthus caryophyllus L.)
Identification of tightly linked SSR markers for flower type in carnation (Dianthus caryophyllus L.)
复制标题
DOI：
10.1007/s10681-014-1090-8
复制
发表时间：
2014-03
期刊：
影响因子：
1.9
通讯作者：
M. Yagi;Toshiya Yamamoto;S. Isobe;S. Tabata;H. Hirakawa;H. Yamaguchi;K. Tanase;T. Onozaki
中科院分区：
文献类型：
--
作者：
M. Yagi;Toshiya Yamamoto;S. Isobe;S. Tabata;H. Hirakawa;H. Yamaguchi;K. Tanase;T. Onozaki
单、重瓣花型是香石竹育种的重要目标之一。我们利用91个F_2群体构建的基于SSR标记的遗传连锁图谱，将控制花型的D85位点定位到LG 85P_15-2上。利用SSR标记作为锚位点进行的位置比较表明，D85位点的图谱位置与控制野生D. capitatusssp.andrzejowskianus.在D85位点上鉴定出4个共分离SSR标记。对4个SSR标记的验证结果表明，其中2个标记（CES 0212和CES 1982）与D85位点紧密连锁，分别扩增出一个176 bp和269 bp的等位基因，这是重瓣花品种所共有的，也是重瓣花品种所特有的。D85位点和紧密连锁SSR标记的定位结果将有助于确定康乃馨花型的遗传基础和标记辅助育种。
Single or double flower type is one of the most important breeding targets in carnation (Dianthus caryophyllusL.). We mapped theD85locus, which controls flower type, to LG 85P_15–2 using a simple sequence repeat (SSR)-based genetic linkage map constructed using 91 F2progeny derived from a cross between line 85–11 (double flower) and ‘Pretty Favvare’ (single flower). A positional comparison using SSR markers as anchor loci revealed that the map positions of theD85locus corresponded to thesinglelocus controlling the single flower type derived from wildD. capitatusssp.andrzejowskianus. We identified four co-segregating SSR markers on theD85locus. Verification of the SSR markers in commercial cultivars revealed that two of the four SSR markers (CES0212 and CES1982) were tightly linked to theD85locus, and amplified a 176-bp and 269-bp allele, respectively, which were common and unique to double flower cultivars. The map positions of theD85locus and the tightly linked SSR markers will be useful for determining the genetic basis of flower type and for marker-assisted breeding of carnations.