Fatty acid synthase is expressed mainly in adult hormone-sensitive cells or cells with high lipid metabolism and in proliferating fetal cells
Fatty acid synthase is expressed mainly in adult hormone-sensitive cells or cells with high lipid metabolism and in proliferating fetal cells
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DOI：
10.1177/002215540004800505
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发表时间：
2000-05-01
影响因子：
3.2
通讯作者：
Suzuki, T
中科院分区：
文献类型：
--
作者：
Kusakabe, T;Maeda, M;Suzuki, T
动物脂肪酸合成酶（FAS）是一种合成长链脂肪酸的同源二聚体蛋白，在肝、脑、乳腺和肺中含量丰富。然而，FAS在人体组织中的精确细胞定位尚未阐明。用一种新的抗人FAS抗体进行免疫组化，结果显示FAS在成人组织中主要分布在高脂代谢细胞中（脂肪细胞、黄体、肝细胞、皮脂腺和II型肺泡细胞），在卵巢敏感细胞中（垂体前叶、顶泌腺、乳腺、子宫内膜、前列腺、精囊和肾上腺皮质），以及在十二指肠和胃的上皮细胞亚群中，结肠吸收细胞、脑神经元、小脑篮状细胞、蜕膜、尿上皮和膀胱。在妊娠20周的胎儿细胞中，FAS主要存在于消化和呼吸系统的增殖上皮细胞、近端肾小管、肾上腺皮质细胞以及间充质和血淋巴样细胞中。染色是显着的非增殖细胞，在成人中观察到的，在交感神经节细胞，睾丸Leidig细胞，绒毛膜绒毛Langhans细胞。FAS在成人中维持在对脂肪酸敏感的细胞和/或在脂质代谢中活跃的细胞中，并且在胎儿中在增殖细胞中表达，表明用于能量利用或膜脂质的活跃脂肪酸合成。
Animal fatty acid synthase (FAS) is a homodimer protein which synthesizes long-chain fatty acids and is rich in liver, brain, breast, and lung. However, the precise cellular localization of FAS in human tissues has not been elucidated. Immunohistochemistry with a new antibody to human FAS revealed that in adult human tissues FAS is distributed mainly in cells with high lipid metabolism (adipocytes, corpus luteum, hepatocytes, sebaceous glands, and Type II alveolar cells), in hormone-sensitive cells (anterior pituitary, apocrine gland, breast, endometrium, prostate, seminal vesicle, and adrenal cortex), and in a subset of epithelial cells of duodenum and stomach, colon absorptive cells, cerebral neurons, basket cells of cerebellum, decidua, uroepithelium, and epidymis. In fetal cells at 20 weeks of gestation, FAS was mainly present in proliferative epithelial cells of the digestive and respiratory systems, proximal renal tubules, adrenocortical cells, and mesenchymal and hematolymphoid cells. Staining was significant in nonproliferating cells, as observed in adult, and in sympathetic ganglion cells, Leidig cells of testis, and Langhans cells of chorionic villi. FAS is maintained in hormone-sensitive cells and/or cells active in lipid metabolism in the adult and is expressed in proliferating cells in the fetus, suggesting active fatty acid synthesis for energy utilization or membrane lipids.