Automated Laboratory Growth Assessment and Maintenance of Azotobacter vinelandii
Automated Laboratory Growth Assessment and Maintenance of Azotobacter vinelandii
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DOI：
10.1002/cpz1.57
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发表时间：
2021-03-01
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影响因子：
--
通讯作者：
Kacar, Betul
中科院分区：
文献类型：
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作者：
Carruthers, Brooke M.;Garcia, Amanda K.;Kacar, Betul
棕色固氮菌Azotobacter vinelandii（A. vinelandii）是一种常用的模式生物，用于研究有氧呼吸作用、几种工业相关化合物的细菌生产以及可能最重要的生物固氮的遗传学和生物化学。实验室生长评估A. vinelandii可用于评估环境和遗传修饰对生理特性（包括固氮）的影响。然而，研究人员通常依赖于手动生长方法，这些方法通常是费力和低效的。我们提出了一个自动生长评估的协议。vinelandii在酶标仪上进行，特别适用于固氮生长的研究。我们讨论了协议优化的常见陷阱和策略，并展示了该协议的应用对固氮基因进行修改的菌株的生长评价。(c)2021年，任作家。
Azotobacter vinelandii (A. vinelandii) is a commonly used model organism for the study of aerobic respiration, the bacterial production of several industrially relevant compounds, and, perhaps most significantly, the genetics and biochemistry of biological nitrogen fixation. Laboratory growth assessments of A. vinelandii are useful for evaluating the impact of environmental and genetic modifications on physiological properties, including diazotrophy. However, researchers typically rely on manual growth methods that are oftentimes laborious and inefficient. We present a protocol for the automated growth assessment of A. vinelandii on a microplate reader, particularly well-suited for studies of diazotrophic growth. We discuss common pitfalls and strategies for protocol optimization, and demonstrate the protocol's application toward growth evaluation of strains carrying modifications to nitrogen-fixation genes. (c) 2021 The Authors.