Apoptotic death in deep partial thickness burns vs. normal skin of burned patients
Apoptotic death in deep partial thickness burns vs. normal skin of burned patients
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DOI：
10.1016/j.jss.2006.07.031
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发表时间：
2007-08-01
影响因子：
2.2
通讯作者：
Montone, Antonio
中科院分区：
文献类型：
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作者：
Gravante, Gianpiero;Palmieri, Maria B.;Montone, Antonio
背景。深度部分烧伤有一个模糊的行为演变为自发愈合或全层烧伤。本研究的目的是研究这些病变是否存在细胞凋亡，从而为其特殊的临床进展提供可能的细胞解释。我们采用DNA片段共定位（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记）和Fas配体CD95抗体计算了21例急性热损伤后深度部分厚度烧伤和正常皮肤的细胞凋亡率（P < 0.05）。深度部分厚度烧伤的细胞凋亡率高于正常皮肤（48.15% +/- 17.22% vs . 18.6% +/- 7.2%; P = 0.0002）。细胞凋亡率与年龄、损伤天数、烧伤总面积、深度烧伤面积均无明显相关性，与性别有轻微相关性（r = 0.484; P = 0.02）。与未烧伤皮肤相比，深度部分厚度烧伤真皮细胞的凋亡率更高。这些数据表明，深度部分厚度的增长源于细胞凋亡。需要具体的研究来证实这一假设，并探讨其临床和治疗意义。(c) 2007爱思唯尔公司版权所有。
Background. Deep partial thickness burns have an ambiguous behavior evolving either into spontaneous healing or full thickness burns. The aim of this study was to investigate these lesions for the presence of apoptosis thereby giving a possible cellular explanation to their peculiar clinical progression.Methods. We used colocalization of DNA fragments (terminal deoxynucleotidyl transferase mediated dUTP nick end labeling) and Fas ligand CD95 antibodies to calculate the apoptotic rate of deep partial thickness burns and normal skin in 21 patients after acute thermal injuries (significant difference considered: P < 0.05).Results. Deep partial thickness burns were associated with a higher apoptotic rate than normal skin (48.15% +/- 17.22% versus 18.6% +/- 7.2%; P = 0.0002). There were no apparent significant correlations of apoptotic rate with age, days from injury, total burn surface area or deep burn area except for a slight correlation with sex (r = 0.484; P = 0.02).Conclusions. A higher apoptotic rate was present in dermal cells of deep partial thickness burns if compared to that of the unburned skin. These data would suggest that deep partial thickness progression derive from apoptosis. Specific studies are required to confirm this hypothesis and to investigate its clinical and therapeutic significance. (c) 2007 Elsevier Inc. All rights reserved.