Titanium nanoparticles potentially affect gingival tissue through IL-13α2 receptor expression
Titanium nanoparticles potentially affect gingival tissue through IL-13α2 receptor expression
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DOI：
10.2334/josnusd.21-0130
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发表时间：
2021-07-01
影响因子：
1.9
通讯作者：
Kondo, Hisatomo
中科院分区：
文献类型：
--
作者：
Ishikawa, Taichi;Sugawara, Shiho;Kondo, Hisatomo
目的：研究钛纳米颗粒对基因和启动子表达及牙龈组织的影响。方法：以Ca9-22细胞系作为牙龈上皮细胞，评价二氧化钛纳米材料作为纳米钛的作用。在基因和启动子表达分析之前，细胞与二氧化钛纳米材料共培养或不共培养。采用实时定量逆转录聚合酶链反应和免疫荧光染色检测白细胞介素-13 α 2受体的表达。此外，采用相同的方法分析了转化生长因子β 1的信使核糖核酸（mRNA）表达的增强。结果：二氧化钛纳米材料影响Ca9-22细胞中基因和启动子的表达：160个上调基因中，编码白介素-13 α 2受体的IL13RA2上调幅度最大（8.625 log(2)倍变化）。免疫荧光染色证实白介素-13 α 2受体表达增加，白介素-13刺激可增强转化生长因子β 1的表达。结论：纳米二氧化钛材料应用于种植体周围牙龈上皮可增加白细胞介素-13 α 2受体的表达。反过来，这可以增强转化生长因子β 1的分泌，而转化生长因子β 1可以促进参与骨吸收的破骨细胞的分化，并可能影响牙龈组织。
Purpose: To determine the effects of titanium nanoparticles, that may have been scattered after dental implant placement, on gene and promoter expression, and gingival tissue.Methods: Ca9-22 cell lines were used as gingival epithelial cells to assess the effects of titanium dioxide nanomaterials as titanium nanoparticles. Cells were cocultured with or without titanium dioxide nanomaterials prior to gene and promoter expression analysis. Expression of interleukin-13 alpha 2 receptor was investigated using real-time quantitative reverse-transcription polymerase chain reaction and immunofluorescence staining. Additionally, the enhanced messenger ribonucleic acid (mRNA) expression of transforming growth factor beta 1 was analyzed using the same method.Results: Titanium dioxide nanomaterials affected gene and promoter expression in Ca9-22 cells: among the 160 upregulated genes, the up-regulation of IL13RA2, which encodes interleukin-13 alpha 2 receptor, was the highest (8.625 log(2) fold change). Immunofluorescence staining confirmed the increased expression of interleukin-13 alpha 2 receptor, which enhanced transforming growth factor beta 1 expression by stimulation with interleukin-13.Conclusion: Titanium dioxide nanomaterials applied on the gingival epithelium around the dental implant may increase interleukin-13 alpha 2 receptor expression. In turn, this can enhance the secretion of transforming growth factor beta 1, which is known to promote the differentiation of osteoclasts involved in bone resorption, and potentially affect gingival tissue.