Streptococcus suis serotypes characterized by analysis of chaperonin 60 gene sequences
Streptococcus suis serotypes characterized by analysis of chaperonin 60 gene sequences
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DOI：
10.1128/aem.67.10.4828-4833.2001
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发表时间：
2001-10-01
影响因子：
4.4
通讯作者：
Hemmingsen, SM
中科院分区：
文献类型：
--
作者：
Brousseau, R;Hill, JE;Hemmingsen, SM
猪链球菌是一种重要的猪病原体，偶尔也会感染人类。已知该菌有35种血清型，需要开发快速的方法来鉴定和区分该菌株。为此，测定了35株猪链球菌血清型参考菌株的伴侣蛋白60部分基因序列。两两距离矩阵分析表明，用最大似然法计算值时，距离范围为0 ~ 0.275。其中5株与血清型ⅰ的距离大于0.1,2株与血清型ⅰ的距离大于0.25，表明它们属于不同的种。大多数核苷酸差异是沉默的；蛋白质序列比对表明，35株菌株只有11个不同的序列。chaperonin 60基因系统发育树与先前发表的基于16S rRNA序列的树相似，并且观察到具有相同chaperonin 60基因序列的菌株往往具有相同的16S rRNA序列。与16S RNA序列相比，伴侣蛋白60基因序列提供了更高水平的血清型区分，可以形成诊断方案的基础。
Streptococcus suis is an important pathogen of swine which occasionally infects humans as well. There are 35 serotypes known for this organism, and it would be desirable to develop rapid methods methods to identify and differentiate the strains of this species. To that effect, partial chaperonin 60 gene sequences were determined for the 35 serotype reference strains of S. suis. Analysis of a pairwise distance matrix showed that the distances ranged from 0 to 0.275 when values were calculated by the maximum-likelihood method. For five of the strains the distances from serotype I were greater than 0.1, and for two of these strains the distances were were more than 0.25, suggesting that they belong to a different species. Most of the nucleotide differences were silent; alignment of protein sequences showed that there were only 11 distinct sequences for the 35 strains under study. The chaperonin 60 gene phylogenetic tree was similar to the previously published tree based on 16S rRNA sequences, and it was also observed that strains with identical chaperonin 60 gene sequences tended to have identical 16S rRNA sequences. The chaperonin 60 gene sequences provided a higher level of discrimination between serotypes than the 16S RNA sequences provided and could form the basis for a diagnostic protocol.