Custom-Size, Functional, and Durable DNA Origami with Design-Specific Scaffolds
Custom-Size, Functional, and Durable DNA Origami with Design-Specific Scaffolds
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DOI：
10.1021/acsnano.9b01025
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发表时间：
2019-05-01
期刊：
影响因子：
17.1
通讯作者：
Dietz, Hendrik
中科院分区：
文献类型：
--
作者：
Engelhardt, Floris A. S.;Praetorius, Florian;Dietz, Hendrik
DNA折纸纳米物体通常是围绕通用单链“支架”设计的。目标对象的许多属性是由那些通用支架序列的细节决定的。在这里，我们使设计人员不仅能够根据所需的 3D 形状，而且根据所使用的序列来完全指定目标结构。为此，我们构建了设计工具来根据链图从头构建支架序列，并开发了可扩展的生产方法，用于使用完全用户定义的序列创建设计特定的支架链。我们使用17个具有不同长度和序列特性的定制支架来研究序列冗余和序列组成对多层DNA折纸组装的影响，并实现多支架DNA折纸物体的高效一锅组装。此外，作为功能化支架的例子，我们创建了一个支架，可以通过紫外线照射实现 DNA 折纸的直接、共价交联，并且我们构建了含有 DNAzyme 的支架，可以允许折叠后 DNA 折纸结构域分离。
DNA origami nano-objects are usually designed around generic single-stranded "scaffolds". Many properties of the target object are determined by details of those generic scaffold sequences. Here, we enable designers to fully specify the target structure not only in terms of desired 3D shape but also in terms of the sequences used. To this end, we built design tools to construct scaffold sequences de novo based on strand diagrams, and we developed scalable production methods for creating design-specific scaffold strands with fully user-defined sequences. We used 17 custom scaffolds having different lengths and sequence properties to study the influence of sequence redundancy and sequence composition on multilayer DNA origami assembly and to realize efficient one-pot assembly of multiscaffold DNA origami objects. Furthermore, as examples for functionalized scaffolds, we created a scaffold that enables direct, covalent cross-linking of DNA origami via UV irradiation, and we built DNAzyme-containing scaffolds that allow postfolding DNA origami domain separation.