Neonatal and adult myosin heavy chain isoforms in a nerve-muscle culture system.
Neonatal and adult myosin heavy chain isoforms in a nerve-muscle culture system.
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DOI：
10.1083/jcb.103.3.995
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发表时间：
1986-09
期刊：
影响因子：
--
通讯作者：
Whalen RG
中科院分区：
文献类型：
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作者：
Ecob-Prince MS;Jenkison M;Butler-Browne GS;Whalen RG
当成年小鼠肌肉纤维与胚胎小鼠脊髓共培养时，肌肉再生以形成肌管，其发展横纹和收缩。我们已经调查了肌球蛋白重链（MHC）亚型存在于这些文化中使用多克隆抗体的新生儿，成人快，慢MHC亚型的大鼠（所有这些都被证明与类似的小鼠亚型特异性反应）在免疫细胞化学测定。成人快速MHC在新形成的肌管中不存在，但在以后的时间被发现，尽管当肌管在没有脊髓组织的情况下培养时不存在。当神经诱导的肌肉收缩被α-银环蛇毒素的持续存在所阻断时，含有成人快速MHC的纤维的比例没有减少。在培养物中始终发现新生和慢MHC，即使在没有脊髓的情况下，因此认为它们的表达不是神经依赖性的。因此，在这种培养系统中，成人快速MHC的表达需要脊髓的存在，但可能不依赖于神经诱导的肌纤维收缩活动。
When adult mouse muscle fibers are co-cultured with embryonic mouse spinal cord, the muscle regenerates to form myotubes that develop cross- striations and contractions. We have investigated the myosin heavy chain (MHC) isoforms present in these cultures using polyclonal antibodies to the neonatal, adult fast, and slow MHC isoforms of rat (all of which were shown to react specifically with the analogous mouse isoforms) in an immunocytochemical assay. The adult fast MHC was absent in newly formed myotubes but was found at later times, although it was absent when the myotubes myotubes were cultured without spinal cord tissue. When nerve-induced muscle contractions were blocked by the continuous presence of alpha-bungarotoxin, there was no decrease in the proportion of fibers that contained adult fast MHC. Neonatal and slow MHC were found at all times in culture, even in the absence of the spinal cord, and so their expression was not thought to be nerve- dependent. Thus, in this culture system, the expression of adult fast MHC required the presence of the spinal cord, but was probably not dependent upon nerve-induced contractile activity in the muscle fibers.