The repertoire of nitrogen assimilation in Rhodococcus : catalysis, pathways and relevance in biotechnology and bioremediation
The repertoire of nitrogen assimilation in Rhodococcus : catalysis, pathways and relevance in biotechnology and bioremediation
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红球菌氮同化作用的全部：催化、途径以及生物技术和生物修复的相关性
DOI：
10.1002/jctb.4321
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发表时间：
2014
影响因子：
3.4
通讯作者：
Foster A
中科院分区：
文献类型：
--
作者：
Foster A
该Rhodococcusgenus表现出不同的酶活性，可以利用在天然和人为的含氮化合物的转化。这种催化反应在可利用的营养物方面提供了选择性优势，同时还用于去除其他有害的外源性物质。这篇综述对有机氮化合物生物转化的文献进行了严格的评估，并考虑了其在生物修复和商业生物技术中的应用。通过依次检查主要的硝基有机化合物（氨基酸，胺，腈，酰胺和硝基芳族化合物），Rhodococcusspp.是成立的。可用的已发表的酶反应数据与基因组表征相结合，为红球菌酶活性提供分子基础，并评估有助于底物可及性和确保稳定性的细胞特性。与所观察到的反应途径相关的代谢基因簇的确定和未来的酶优化和代谢工程的方向进行评估。© 2014化学工业协会。
TheRhodococcusgenus exhibits diverse enzymatic activity that can be exploited in the conversion of natural and anthropogenic nitrogenous compounds. This catalytic response provides a selective advantage in terms of available nutrients while also serving to remove otherwise harmful xenobiotics. This review provides a critical assessment of the literature on bioconversion of organo‐nitrogen compounds with a consideration of applications in bioremediation and commercial biotechnology. By examining the major nitro‐organic compounds (amino acids, amines, nitriles, amides and nitroaromatics) in turn, the considerable repertoire ofRhodococcusspp. is established. The available published enzyme reaction data is coupled with genomic characterisation to provide a molecular basis forRhodococcusenzyme activity with an assessment of the cellular properties that aid substrate accessibility and ensure stability. The metabolic gene clusters associated with the observed reaction pathways are identified and future directions in enzyme optimisation and metabolic engineering are assessed. © 2014 Society of Chemical Industry.