Single-cell RNA-seq reveals cell type-specific transcriptional signatures at the maternal-foetal interface during pregnancy.
Single-cell RNA-seq reveals cell type-specific transcriptional signatures at the maternal-foetal interface during pregnancy.
复制标题
DOI：
10.1038/ncomms11414
复制
发表时间：
2016-04-25
影响因子：
16.6
通讯作者：
Robertson EJ
中科院分区：
文献类型：
--
作者：
Nelson AC;Mould AW;Bikoff EK;Robertson EJ
哺乳动物胚胎在子宫环境中的生长和存活取决于胎盘，胎盘是由母体和胎儿组织组成的高度复杂的血管化器官。最近的实验表明，锌指转录抑制蛋白Prdm 1/Blimp 1是必不可少的规范螺旋动脉滋养层巨细胞（SpA-TGC）入侵和重塑母体血管。为了了解更多关于Blimp 1+细胞谱系的功能贡献，我们在这里进行了胎盘的第一个单细胞RNA-seq分析。分析胎儿和母体来源的细胞类型。与突变胎盘和体外分化的滋养层干细胞的微阵列数据集的比较，使我们能够确定Blimp 1依赖的转录富集SpA-TGCs。我们的实验提供了新的见解功能不同的细胞类型存在于母胎界面和推进我们的知识的动态基因表达模式控制胎盘形态发生和血管拟态。 锌指转录抑制因子Prdm 1/Blimp 1对滋养层细胞亚群中母体血管重塑至关重要。在这里，作者对这种Blimp 1+谱系进行了单细胞RNA-seq分析，鉴定了存在于母体-胎儿界面的功能不同的细胞类型。
Growth and survival of the mammalian embryo within the uterine environment depends on the placenta, a highly complex vascularized organ comprised of both maternal and foetal tissues. Recent experiments demonstrate that the zinc finger transcriptional repressor Prdm1/Blimp1 is essential for specification of spiral artery trophoblast giant cells (SpA-TGCs) that invade and remodel maternal blood vessels. To learn more about functional contributions made by Blimp1+ cell lineages here we perform the first single-cell RNA-seq analysis of the placenta. Cell types of both foetal and maternal origin are profiled. Comparisons with microarray datasets from mutant placenta and in vitro differentiated trophoblast stem cells allow us to identify Blimp1-dependent transcripts enriched in SpA-TGCs. Our experiments provide new insights into the functionally distinct cell types present at the maternal–foetal interface and advance our knowledge of dynamic gene expression patterns controlling placental morphogenesis and vascular mimicry. The zinc finger transcriptional repressor Prdm1/Blimp1 is essential for remodelling maternal blood vessels in a subset of trophoblast cells. Here, the authors perform single-cell RNA-seq analysis on this Blimp1+ lineage, identifying functionally distinct cell types present at the maternal–foetal interface.