Microdevice with integrated Clark-type oxygen electrode for measurement of respiratory activity of cells
Microdevice with integrated Clark-type oxygen electrode for measurement of respiratory activity of cells
复制标题
具有集成克拉克型氧电极的微型装置，用于测量细胞的呼吸活动
DOI：
10.1021/acs.analchem.1c00227
复制
发表时间：
2021
影响因子：
7.4
通讯作者：
Suzuki H
中科院分区：
文献类型：
--
作者：
Hsueh AJ;Park S;Satoh T;Shimizu T;Koiwai K;Nakashima M;Morimoto Y;Kinoshita M;Suzuki H
采用微制造的克拉克型氧电极，制作了一种用于测量细胞呼吸活动的微型装置。氧电极在干燥状态下完成，并通过透氧膜引入水蒸气形式的氧气还原所需的水来激活，这大大方便了即使是非专业人员也能操作该设备。薄纸层的使用稳定了电流响应，并使氧电极稳定连续运行，而不受水蒸发引起的电流干扰。该装置在一些模型实验中进行了测试，包括测量大肠杆菌的呼吸活性。大肠杆菌)、抗生素疗效的评估和中性粒细胞抗菌活性的测量，所有这些都表明，通过遵循一个简单的测量准备程序，可以清楚地监测细胞对溶解氧的消耗。
A microdevice for the measurement of the respiratory activity of cells was fabricated using a microfabricated Clark-type oxygen electrode. The oxygen electrode was completed in a dry state and was activated by introducing water necessary for the reduction of oxygen in the form of water vapor through an oxygen-permeable membrane, which significantly facilitated handling of the device even by nonspecialists. The use of a thin paper layer stabilized the current response and enabled stable continuous operation of the oxygen electrode without current disturbance caused by the evaporation of water. The microdevice was tested in some model experiments including the measurement of the respiratory activity ofEscherichia coli(E. coli), evaluation of the efficacy of antibiotics, and measurement of the antibacterial activity of neutrophils, all of which demonstrated that the consumption of dissolved oxygen by cells can be monitored clearly by following an easy procedure for the preparation of the measurements.