Expression of carbonic anhydrase II (CA II) promoter-reporter fusion genes in multiple tissues of transgenic mice does not replicate normal patterns of expression indicating complexity of CA II regulation in vivo.
Expression of carbonic anhydrase II (CA II) promoter-reporter fusion genes in multiple tissues of transgenic mice does not replicate normal patterns of expression indicating complexity of CA II regulation in vivo.
复制标题
转基因小鼠多个组织中碳酸酐酶 II (CA II) 启动子-报告基因融合基因的表达不能复制正常的表达模式，表明 CA II 体内调节的复杂性。
DOI：
10.1007/bf00554600
复制
发表时间：
1995
影响因子：
2.4
通讯作者：
Tashian,RE
中科院分区：
文献类型：
--
作者：
Erickson,RP;Grimes,J;Venta,PJ;Tashian,RE
虽然人碳酸氢酶II(CA II)基因近端5‘115bp的片段足以在某些转基因细胞系中表达报告基因，但我们先前发现该启动子的1100bp(或小鼠CA II启动子的500bp)不足以在转基因小鼠中表达。我们现在已经研究了报告基因在转基因小鼠中的表达：(1)11kb的人5‘启动子或(2)8kb的人5’启动子与小鼠的第一外显子、第一内含子的一部分(因为CpG岛横跨该区域)以及该基因的3‘序列。虽然两种方法的敏感性不同，但第一种构建物在许多组织中都有表达，而第二种构建体仅在脾组织中表达。这些发现表明，体内CA II表达的DNA控制区相当复杂。
Although the proximal, 5′ 115 bp of the human carbonic anhydrase II (CA II) gene was sufficient for expression of a reporter gene in some transfected cell lines, we found previously that 1100 bp of this promoter (or 500 bp of the mouse CA II promoter) was not sufficient for expression in transgenic mice. We have now studied the expression of linked reporter genes in mice transgenic for either (1) 11 kb of the human 5′ promoter or (2) 8 kb of the human 5′ promoter with mouse sequences from the first exon, part of the first intron (since a CpG island spans this region), and the 3′ sequences of the gene. Expression was found in both cases, but the tissue specificity was not appropriate for CA II. Although there was a difference in the sensitivity of the assays used, the first construct led to expression in many tissues, while the second construct was expressed only in spleen. These findings indicate considerable complexity of DNA control regions for in vivo CA II expression.