Stability of West Nile Virus (Flaviviridae: Flavivirus) RNA in Mosquito Excreta
Stability of West Nile Virus (Flaviviridae: Flavivirus) RNA in Mosquito Excreta
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蚊子排泄物中西尼罗病毒（黄病毒科：黄病毒）RNA 的稳定性
DOI：
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发表时间：
2019
影响因子：
2.1
通讯作者：
A. F. van den Hurk
中科院分区：
文献类型：
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作者：
A. L. Ramírez;Sonja Hall;G. Hewitson;J. McMahon;K. Staunton;S. Ritchie;A. F. van den Hurk
摘要虫媒病毒监测是实施病媒传染病控制措施的关键。最近，它已被证明，传播虫媒病毒感染的蚊子分泌病毒RNA，这可以通过分子方法检测。因此，蚊子排泄物已被提议作为可用于虫媒病毒监测的样品类型。在这项研究中，我们评估，如果西尼罗河病毒（Kunjin株，WNVKUN）的RNA在库蚊annulirostris Skuse（双翅目：蚊科）排泄物沉积在不同的基板上储存长达2周后，在热带高温高湿条件下可以检测到。在14天内，未观察到沉积在Flinders Associate Technologies（FTA）卡上的排泄物中WNVKUN RNA的相对量（通过比较Ct值确定）显著下降，表明RNA在该时间内稳定。24 h后，沉积在FTA卡或聚碳酸酯底物上的排泄物中WNVKUN RNA的相对量无显著差异。然而，7和14天后，有一个显着的下降，在排泄物中的病毒RNA的相对数量存储在聚碳酸酯基板上。对于纳入虫媒病毒监测计划，我们建议使用聚碳酸酯基板的排泄物收集蚊子陷阱部署过夜，并在陷阱中的FTA卡每周或每两周服务的集成。聚碳酸酯基质有利于收集来自诱捕器的大部分排泄物，虽然FTA卡提供有限的区域覆盖，但它们能够在热带条件下长时间保存病毒RNA。
Abstract Arbovirus surveillance is crucial for the implementation of vector-borne disease control measures. Recently, it has been demonstrated that mosquitoes with a disseminated arbovirus infection excrete viral RNA, which can be detected by molecular methods. Thereby, mosquito excreta has been proposed as a sample type that could be utilized for arbovirus surveillance. In this study, we evaluated if West Nile virus (Kunjin strain, WNVKUN) RNA in Culex annulirostris Skuse (Diptera: Culicidae) excreta deposited on different substrates could be detected after storage for up to 2 wk at tropical conditions of high heat and humidity. No significant drop in relative quantity of WNVKUN RNA (determined by comparison of Ct values) in excreta deposited on Flinders Associate Technologies (FTA) cards was observed over 14 d, suggesting that RNA was stable for that time. There was no significant difference in relative quantity of WNVKUN RNA in excreta deposited on FTA cards or polycarbonate substrates after 24 h. However, after 7 and 14 d, there was a significant decline in the relative quantity of viral RNA in the excreta stored on polycarbonate substrates. For incorporation in arbovirus surveillance programs, we recommend the use of polycarbonate substrates for excreta collection in mosquito traps deployed overnight, and the integration of FTA cards in traps serviced weekly or fortnightly. Polycarbonate substrates facilitate the collection of the majority of excreta from a trap, and while FTA cards offer limited area coverage, they enable preservation of viral RNA in tropical conditions for extended periods of time.