Inhibitory effects of the standardized extract (DA-9601) of Artemisia asiatica Nakai on phorbol ester-induced ornithine decarboxylase activity, papilloma formation, cyclooxygenase-2 expression, inducible nitric oxide synthase expression and nuclear transcription factor κB activation in mouse skin
Inhibitory effects of the standardized extract (DA-9601) of Artemisia asiatica Nakai on phorbol ester-induced ornithine decarboxylase activity, papilloma formation, cyclooxygenase-2 expression, inducible nitric oxide synthase expression and nuclear transcription factor κB activation in mouse skin
复制标题
DOI：
10.1002/ijc.10489
复制
发表时间：
2002-08-01
影响因子：
6.4
通讯作者：
Surh, YJ
中科院分区：
文献类型：
--
作者：
Seo, HJ;Park, KK;Surh, YJ
亚洲蒿已被用于传统的亚洲医学，用于治疗炎症和其他疾病。以往的研究表明，A.积雪草具有显著的抗氧化和抗氧化活性，并对实验诱导的胃肠道、肝和胰腺损伤表现出细胞保护作用。在本研究中，我们评估了DA-9601对肿瘤促进的抑制作用，这与炎症组织损伤密切相关。作为评价DA-9601可能的抗肿瘤促进潜力的初步方法，在雌性ICR小鼠中检查了其对TPA诱导的耳水肿的影响。在局部应用TPA前30 min用DA-9601预处理小鼠耳内表面可抑制5 h耳水肿。TPA刺激的表皮考克斯-2和NOS表达也可通过局部应用相同的提取物减轻。此外，DA-9601消除了TPA介导的小鼠表皮中NF-κ B/Rel和AP-1的活化。DA-9601对表皮NF-κ B的抑制似乎部分通过抑制IkappaB α降解介导，从而阻断NF-κ B功能亚基p65的核转位。DA-9601还显著抑制TPA诱导的ODC活性和小鼠皮肤中的乳头状瘤形成。综上所述，这些结果表明DA-9601来源于A.积雪草具有潜在的化学预防活性。(C)2002 Wiley-Liss，Inc.
Artemisia asiatica Nakai has been used in traditional Asian medicine for the treatment of inflammatory and other disorders. Previous studies have revealed that the formulated ethanol extract (DA-9601) of A. asiatica has pronounced antioxidative and antiinflammatory activities and exhibits cytoprotective effects against experimentally induced gastrointestinal, hepatic and pancreatic damage. In the present study, we assessed the inhibitory effect of DA-9601 on tumor promotion, which is closely linked to inflammatory tissue damage. As an initial approach to evaluating the possible antitumor-promoting potential of DA-9601, its effects on TPA-induced ear edema were examined in female ICR mice. Pretreatment of the inner surface of the mouse ear with DA-9601 30 min prior to topical application of TPA inhibited ear edema at 5 hr. TPA-stimulated expression of epidermal COX-2 and NOS was also mitigated by topical application of the same extract. Moreover, DA-9601 abrogated the TPA-mediated activation of NF-kappaB/Rel and AP-I in mouse epidermis. Suppression of epidermal NF-kappaB by DA-9601 appeared to be mediated in part through inhibition of IkappaBalpha degradation, thereby blocking the nuclear translocation of p65, the functional subunit of NF-kappaB. DA-9601 also significantly suppressed TPA-induced ODC activity and papilloma formation in mouse skin. Taken together, these findings suggest that DA-9601 derived from A. asiatica possesses potential chemopreventive activities. (C) 2002 Wiley-Liss, Inc.