Stimulation of DNA-dependent protein kinase activity by high mobility group proteins 1 and 2.
Stimulation of DNA-dependent protein kinase activity by high mobility group proteins 1 and 2.
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高迁移率族蛋白 1 和 2 刺激 DNA 依赖性蛋白激酶活性。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
H. Teraoka
中科院分区：
文献类型：
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作者：
F. Watanabe;H. Shirakawa;M. Yoshida;K. Tsukada;H. Teraoka
在双链 DNA 存在下，将高迁移率基团 (HMG) 蛋白 1 和 2 与 DNA 依赖性蛋白激酶 (DNA-PK) 和 [gamma-32P]ATP 一起孵育后，不仅观察到 HMG 蛋白的磷酸化，而且观察到 DNA-PK 中 350 kDa 催化多肽的自身磷酸化增强。以合成肽和 α-酪蛋白为底物测定的 DNA-PK 活性被 HMG1、HMG2 和 DNA 结合域刺激数倍。 HMG 蛋白浓度较高时，刺激作用减弱，并且组蛋白 H1 抑制 DNA-PK 活性。电泳迁移率变动分析表明 HMG 蛋白促进 DNA-PK 与 DNA 的结合。
After incubation of high mobility group (HMG) proteins 1 and 2 with DNA-dependent protein kinase (DNA-PK) and [gamma-32P]ATP in the presence of double-stranded DNA, not only phosphorylation of HMG proteins but also enhancement of autophosphorylation of the catalytic polypeptide of 350 kDa in DNA-PK was observed. DNA-PK activity determined with a synthetic peptide and alpha-casein as substrates was stimulated several-fold by HMG1, HMG2, and the DNA-binding domains. The stimulation was decreased at higher concentrations of HMG proteins, and DNA-PK activity was inhibited by histone H1. Electrophoretic mobility shift analysis suggests that HMG proteins facilitate the binding of DNA-PK to DNA.