Indirect evidence for enhancement of Na-K-ATPase activity with stimulation of rectal gland secretion.
Indirect evidence for enhancement of Na-K-ATPase activity with stimulation of rectal gland secretion.
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通过刺激直肠腺分泌来增强 Na-K-ATP 酶活性的间接证据。
DOI：
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发表时间：
1979
影响因子：
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通讯作者：
F. Epstein
中科院分区：
文献类型：
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作者：
P. Silva;J. Stoff;F. Epstein
在cAMP和茶碱刺激下，研究了棘角鲨离体灌流直肠腺活性氯离子分泌与na - k - atp酶活性的关系。瓦巴因抑制受刺激腺体的分泌和摄氧量。cAMP和茶碱刺激氯离子分泌导致乌阿巴因抑制的耗氧量增加6 - 7倍。细胞内钠浓度降低，细胞内钾浓度升高。这些发现提示na - k - atp酶的激活。另一方面，经cAMP刺激灌注腺体后，直肠组织全匀浆中na - k - atp酶活性没有增加，瓦巴因结合也没有增加。刺激分泌后计算的细胞内氯化物浓度下降，与cAMP增加腔内对氯化物的通透性的作用一致。
The relation between active chloride secretion and Na-K-ATPase activity was studied in the isolated perfused rectal gland of Squalus acanthias stimulated by cAMP and theophylline. Ouabain inhibited both secretion and oxygen uptake in stimulated glands. Stimulation of chloride secretion by cAMP and theophylline resulted in a six- to sevenfold rise in ouabain-inhibitable oxygen consumption. Intracellular sodium concentration decreased while intracellular potassium rose. These findings suggest activation of Na-K-ATPase. On the other hand, the activity of Na-K-ATPase in whole homogenates of rectal gland tissue was not increased after cAMP stimulation of the perfused gland, nor was ouabain binding increased. Calculated intracellular chloride concentration fell after secretion was stimulated in a way consistent with an action of cAMP to increase luminal permeability to chloride.