DNA CLONING OF PLASMODIUM-FALCIPARUM CIRCUMSPOROZOITE GENE - AMINO-ACID-SEQUENCE OF REPETITIVE EPITOPE
DNA CLONING OF PLASMODIUM-FALCIPARUM CIRCUMSPOROZOITE GENE - AMINO-ACID-SEQUENCE OF REPETITIVE EPITOPE
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DOI：
10.1126/science.6204384
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
NUSSENZWEIG, RS
中科院分区：
文献类型：
--
作者：
ENEA, V;ELLIS, J;NUSSENZWEIG, RS
通过用 CS 蛋白的单克隆抗体筛选大肠杆菌互补 DNA 文库，分离出编码人类疟原虫恶性疟原虫的环子孢子 (CS) 蛋白的互补 DNA 克隆。互补DNA插入片段的DNA序列编码4个氨基酸序列； Pro-Asn-Ala-Asn，串联重复23次。 CSβ-内酰胺酶融合蛋白将单克隆抗体与CS蛋白特异性结合，并抑制这些抗体与天然恶性疟原虫CS蛋白的结合。这些发现为开发针对恶性疟原虫疟疾的疫苗提供了基础。
A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite P. falciparum was isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a 4-amino acid sequence; Pro-Asn-Ala-Asn, tandemly repeated 23 times. The CS .beta.-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native P. falciparum CS protein. These findings provide a basis for the development of a vaccine against P. falciparum malaria.