Glucosyltransferase activity of Clostridium difficile Toxin B is essential for disease pathogenesis.
Glucosyltransferase activity of Clostridium difficile Toxin B is essential for disease pathogenesis.
复制标题
艰难梭菌毒素 B 的葡萄糖基转移酶活性对于疾病发病机制至关重要。
DOI：
10.1080/19490976.2015.1062965
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发表时间：
2015
期刊：
影响因子：
12.2
通讯作者：
Feng,Hanping
中科院分区：
文献类型：
--
作者：
Yang,Zhiyong;Zhang,Yongrong;Huang,Tuxiong;Feng,Hanping
Clostridium difficileTcdB harbors a glucosyltransferase that targets host Rho GTPases. However, the role of the enzyme activity in the induction of host intestinal disease has not been demonstrated. In this study, we established a mouse acute intestinal disease model by cecum injection of wild type and glucosyltransferase-deficient TcdB and a chronic model by delivering toxin intraluminally via engineered surrogate hostBacillus megaterium. We demonstrated, for the first time, that the glucosyltransferase activity of TcdB is essential for inducing disease symptoms and intestinal pathological responses that resemble human disease, highlighting the importance of targeting toxin glucosyltransferase activity for future therapy.
