Seasonality and monthly dynamics of marine myovirus communities
Seasonality and monthly dynamics of marine myovirus communities
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DOI：
10.1111/j.1462-2920.2012.02744.x
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发表时间：
2012-08-01
影响因子：
5.1
通讯作者：
Fuhrman, Jed A.
中科院分区：
文献类型：
--
作者：
Chow, Cheryl-Emiliane T.;Fuhrman, Jed A.
海洋肌病毒（即具有可收缩尾鞘的噬菌体）数量丰富且遗传多样。我们开发了一种末端限制性片段长度多态性分析（TRFLP）的g23，保守的基因编码的主要衣壳蛋白，调查T4样肌病毒社区在南加州大学的微生物天文台在圣佩德罗海洋时间序列（SPOT），在那里我们以前报告了细菌的季节性。在3年中，每月观察到71至154个操作分类单位（OTU）。大约25%的OTU是在31个月或更长时间内检测到的。T4样肌病毒群落结构随季节变化，一些OTU在春夏反复达到峰值，另一些在秋冬，而适度丰富的OTU全年持续存在。使用判别函数分析（DFA，选择最能预测月份的分类群）和平均BrayCurtis相似性证明了重复的群落结构。DFA分析表明，相邻月份或相隔12个月的社区之间存在正自相关，而相隔37个月的社区之间存在负自相关。BrayCurtis相似性是最高的相邻月份之间的局部最大值在12个月和局部最小值在6和18至20个月的滞后。SPOT的T4样病毒群落表现出季节性，然而，适度丰富的OTU和群落的可预测性的某种意想不到的持久性为现有的海洋病毒概念模型增加了新的曲折。
Marine myoviruses (i.e. bacteriophages with a contractile tail sheath) are numerically abundant and genetically diverse. We developed a terminal restriction fragment length polymorphism assay (TRFLP) for g23, the conserved gene encoding the major capsid protein, to investigate T4-like myovirus communities at USC's Microbial Observatory at the San Pedro Ocean Time-series (SPOT), where we previously reported bacterial seasonality. Between 71 and 154 operational taxonomic units (OTUs) were observed monthly over 3 years. Roughly 25% of OTUs were detected in 31 or more months. T4-like myoviral community structure varied seasonally with some OTUs peaking repeatedly in springsummer and others in fallwinter, while moderately abundant OTUs persisted year-round. Recurring community structure was demonstrated using discriminant function analysis (DFA, selecting taxa that best predict months) and average BrayCurtis similarity. DFA showed communities from adjacent months or 12 months apart were positively auto-correlated, while communities 37 months apart were negatively auto-correlated. BrayCurtis similarity was highest between adjacent months with a local maximum at 12-month and local minima at 6- and 18- to 20-month lags. The T4-like virus community at SPOT exhibited seasonality, yet the somewhat unexpected persistence of moderately abundant OTUs and predictability of the community add new twists to existing conceptual models of marine viruses.