Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung
Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung
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DOI：
10.3791/50601
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发表时间：
2013-11-01
影响因子：
1.2
通讯作者：
Kimura, Shioko
中科院分区：
文献类型：
--
作者：
Cai, Yan;Kimura, Shioko
使用模拟人类肺部疾病状况的模型对于研究特定疾病的病理生理学和/或病因学以及开发治疗干预措施至关重要。随着基因敲除和转基因衍生品的日益普及，以及大量的遗传信息，小鼠提供了研究肺部疾病病理和生理基础的分子机制的最佳模型之一。吸入、鼻内滴注、气管内滴注和气管内插管是许多研究人员在给药小鼠肺部感兴趣的物质时最广泛使用的技术。根据研究目标的不同，每种技术都有优缺点。本文介绍了一种非侵入性的气管内插管方法，可将外源性物质直接输送到小鼠肺内。将该技术应用于小鼠肺内给药，作为研究肺纤维化的模型。
The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. With the increasing availability of knockout and transgenic derivatives, together with a vast amount of genetic information, mice provide one of the best models to study the molecular mechanisms underlying the pathology and physiology of lung diseases. Inhalation, intranasal instillation, intratracheal instillation, and intratracheal intubation are the most widely used techniques by a number of investigators to administer materials of interest to mouse lungs. There are pros and cons for each technique depending on the goals of a study. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented. This technique was applied to administer bleomycin to mouse lungs as a model to study pulmonary fibrosis.