TRBO: A high-efficiency tobacco mosaic virus RNA-Based overexpression vector
TRBO: A high-efficiency tobacco mosaic virus RNA-Based overexpression vector
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DOI：
10.1104/pp.107.106377
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发表时间：
2007-12-01
期刊：
影响因子：
7.4
通讯作者：
Lindbo, John A.
中科院分区：
文献类型：
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作者：
Lindbo, John A.
瞬时表达是在植物中产生目的蛋白的一种快速、有用的方法。基于烟草花叶病毒（TMV）的瞬时表达载体可以在植物中表达非常高水平的外源蛋白。然而，TMV 载体通常不能通过农杆菌感染有效地递送至植物细胞。经确定，使用缺乏 TMV 外壳蛋白基因序列的 35S 启动子驱动的 TMV 复制子，农杆菌感染非常有效。这种外壳蛋白缺失载体作为瞬时表达系统具有几个有用的特征，包括改进的易用性、更高的蛋白质表达率和改进的生物防护。使用该 TMV 表达载体，一些外源蛋白的表达水平为 3 至 5 mg/g 植物组织鲜重。有人建议，这种新的瞬时表达载体将成为在植物中表达重组蛋白以用于研究或生产目的的有用工具。
Transient expression is a rapid, useful approach for producing proteins of interest in plants. Tobacco mosaic virus (TMV)-based transient expression vectors can express very high levels of foreign proteins in plants. However, TMV vectors are, in general, not efficiently delivered to plant cells by agroinfection. It was determined that agroinfection was very efficient with a 35S promoter-driven TMV replicon that lacked the TMV coat protein gene sequence. This coat protein deletion vector had several useful features as a transient expression system, including improved ease of use, higher protein expression rates, and improved biocontainment. Using this TMV expression vector, some foreign proteins were expressed at levels of 3 to 5 mg/g fresh weight of plant tissue. It is proposed that this new transient expression vector will be a useful tool for expressing recombinant proteins in plants for either research or production purposes.