An electrodiffusion model for effects of surface glycocalyx layer on microvessel permeability
An electrodiffusion model for effects of surface glycocalyx layer on microvessel permeability
复制标题
DOI：
10.1152/ajpheart.00467.2002
复制
发表时间：
2003-04-01
影响因子：
4.8
通讯作者：
Chen, WH
中科院分区：
文献类型：
--
作者：
Fu, BMM;Chen, B;Chen, WH
为了研究内皮表面糖萼对微血管通透性的电荷效应，我们扩展了 Fu 等人开发的三维模型。 (J Biomech Eng 116:502-513, 1994)，内皮间裂在裂口入口处包括带负电的糖萼层。在糖萼层内和界面处考虑了带电溶质的静电和空间排斥。假设糖萼层有四种电荷密度分布。我们的模型表明，微血管壁（包括表面糖萼层和裂口区域）的整体溶质渗透性与电荷密度分布无关，只要它们具有相同的最大值和相同的总电荷。根据实验数据，该模型预测青蛙肠系膜毛细血管糖萼的电荷密度为25-35 meq/l。该模型的一个有趣的预测是，当由于带负电的蛋白质的存在而导致管腔中阳离子和阴离子的浓度不相等时，带负电的糖萼对带正电的溶质比对带负电的溶质提供更大的抵抗力。
To investigate the charge effect of the endothelial surface glycocalyx on microvessel permeability, we extended the three-dimensional model developed by Fu et al. (J Biomech Eng 116: 502-513, 1994) for the interendothelial cleft to include a negatively charged glycocalyx layer at the entrance of the cleft. Both electrostatic and steric exclusions on charged solutes were considered within the glycocalyx layer and at the interfaces. Four charge-density profiles were assumed for the glycocalyx layer. Our model indicates that the overall solute permeability across the microvessel wall including the surface glycocalyx layer and the cleft region is independent of the charge-density profiles as long as they have the same maximum value and the same total charge. On the basis of experimental data, this model predicts that the charge density would be 25-35 meq/l in the glycolcalyx of frog mesenteric capillaries. An intriguing prediction of this model is that when the concentrations of cations and anions are unequal in the lumen due to the presence of negatively charged proteins, the negatively charged glycocalyx would provide more resistance to positively charged solutes than to negatively charged ones.