Analysis of PCP defects in mammalian eye lens.
Analysis of PCP defects in mammalian eye lens.
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DOI：
10.1007/978-1-61779-510-7_12
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发表时间：
2012
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影响因子：
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通讯作者：
McAvoy, John W
中科院分区：
文献类型：
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作者：
Sugiyama, Yuki;McAvoy, John W
多细胞组织和器官通常显示平面细胞极性（PCP），其中组成细胞沿轴沿着排列以形成协调模式。哺乳动物晶状体主要由上皮细胞衍生的纤维细胞组成，这些纤维细胞表现出高度有序的排列，并受PCP信号转导的调节。每个纤维细胞有一个位于顶端的初级纤毛，在大多数情况下，这是极化的透镜前极。在这里，我们描述了如何可视化的全球细胞排列的透镜纤维细胞通过检查缝合模式，是由纤维的尖端在前极会议形成。我们还描述了一种方法，为整个安装准备，允许观察初级纤毛的偏振分布在顶端表面的透镜纤维。鉴于其相对简单，至少在细胞方面，其要求在细胞排列和方向的高度精确度，我们预测，透镜将是一个很好的模型系统，以帮助阐明纤毛和PCP组件在组织和器官的三维组织的发展中的作用。
Multicellular tissues and organs often show planar cell polarity (PCP) where the constituent cells align along an axis to form coordinated patterns. Mammalian eye lenses are mainly comprised of epithelial-derived fiber cells which exhibit highly ordered alignment that is regulated by PCP signaling. Each fiber cell has an apically situated primary cilium and in most cases this is polarized towards the lens anterior pole. Here we describe how to visualize the global cellular alignment of lens fiber cells by examining the suture pattern that is formed by the tips of fibers meeting at the anterior pole. We also describe a method for whole mount preparation which allows observation of the polarized distribution of primary cilia at the apical surface of lens fibers. Given its relative simplicity, at least in cellular terms, and its requirement for a high degree of precision in cellular alignment and orientation, we predict that the lens will be an excellent model system to help elucidate the role of cilia and PCP components in development of three-dimensional organization in tissues and organs.