CHARACTERIZATION OF MELANIN-CONCENTRATING HORMONE FROM RAT HYPOTHALAMUS
CHARACTERIZATION OF MELANIN-CONCENTRATING HORMONE FROM RAT HYPOTHALAMUS
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DOI：
10.1210/endo-125-3-1660
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发表时间：
1989-09-01
期刊：
影响因子：
4.8
通讯作者：
VALE, W
中科院分区：
文献类型：
--
作者：
VAUGHAN, JM;FISCHER, WH;VALE, W
大鼠下丘脑经酸提取、凝胶过滤、抗鲑鱼血清免疫亲和层析、十八烷基柱二步高效液相色谱分离得到一种黑色素浓缩素样肽。分离了几个免疫反应区，气相测序仪的Edman降解表明所有区的氨基酸序列都是相同的。大鼠下丘脑MCH是一种非十八肽，与鲑鱼MCH不同，它的N端延伸了两个氨基酸和四个额外的取代基。大鼠妇幼保健器具有以下初级结构：Asp-Phe-Asp-Met-Leu-Arg-Cys-Met-Leu-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Gln-Val.
A melanin-concentrating hormone (MCH)-like peptide was isolated from rat hypothalamus by acid extraction, gel filtration chromatography, immunoaffinity chromatography using antiserum directed against salmon MCH, and two steps of HPLC using octadecyl columns. Several zones of immunoreactivity were isolated, and Edman degradation in a gas phase sequencer indicated that the amino acid sequence of all zones was identical. Rat hypothalamic MCH is a nonadecapeptide which differs from salmon MCH by an N-terminal extension of two amino acids and four additional substitutions. Rat MCH has the following primary structure: Asp-Phe-Asp-Met-Leu-Arg-Cys-Met-Leu-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Gln-Val.