Transport behavior of ellagic acid of pomegranate leaf tannins and its correlation with total cholesterol alteration in HepG2 cells
Transport behavior of ellagic acid of pomegranate leaf tannins and its correlation with total cholesterol alteration in HepG2 cells
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石榴叶鞣质鞣花酸在HepG2细胞中的转运行为及其与总胆固醇变化的相关性
DOI：
10.1002/bmc.1150
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发表时间：
2009-05-01
影响因子：
1.8
通讯作者：
Du, Lijun
中科院分区：
文献类型：
--
作者：
Lan, Jiaqi;Lei, Fan;Du, Lijun
研究石榴叶单宁中鞣花酸能否被转运到HepG 2细胞中及其转运行为。采用高效液相色谱法，996光电二极管阵列检测器，检测波长254 nm。移动的相为乙腈-水溶液（含0.1%三乙胺，pH 3.0; 16：64，v/v，用于测定细胞中的鞣花酸）。流速为0.8 mL/min。将细胞与100和50 μ g/mL石榴叶单宁（分别含有1.71和0.85 μ g/mL鞣花酸）孵育特定时间，然后在甲醇中裂解和超声处理以提取细胞内鞣花酸。将10 μ L等分试样的样品注入HPLC系统中以测定鞣花酸浓度。结果表明，石榴叶单宁中的鞣花酸可被转运到细胞内，并与细胞内总胆固醇的变化相关。这是首次全面证明鞣花酸在体外通过HepG 2细胞的转运行为。版权所有（C）2008约翰威利父子有限公司
The aim of this study was to investigate whether ellagic acid in pomegranate leaf tannins could be transported into HepG2 cells and its transport behavior. High-performance liquid chromatography coupled-with a 996 photodiode array detector at 254 nm was applied. The mobile phase was an acetonitrile-water solution (containing 0.1% triethylamine, pH 3.0; 16:64, v/v, for determining ellagic acid in cells). The flow rate was 0.8 mL/min. Cells were incubated with pomegranate leaf tannins with 100 and 50 mu g/mL (containing 1.71 and 0.85 mu g/mL of ellagic acid, respectively) for a specific time, then lysed and sonicated in methanol to extract intracellular ellagic acid. A 10 mu L aliquot of sample was injected into the HPLC system to determine ellagic acid concentration. The results showed that ellagic acid in pomegranate leaf tannins could be transported into the cells, which was in correlation with total cholesterol alteration in the cells. This is the first time that the transport behavior of ellagic acid through HepG2 cells in vitro has been comprehensively demonstrated. Copyright (C) 2008 John Wiley & Sons, Ltd.