Salvia miltiorrhiza Injection Promotes the Adipogenic Differentiation of Human Adipose-Derived Stem Cells
Salvia miltiorrhiza Injection Promotes the Adipogenic Differentiation of Human Adipose-Derived Stem Cells
复制标题
丹参注射液促进人脂肪干细胞的成脂分化
DOI：
10.1097/prs.0000000000007671
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发表时间：
2021-03-01
影响因子：
3.6
通讯作者：
An, Juan
中科院分区：
文献类型：
--
作者：
Lin, Huaian;Zhang, Yifan;An, Juan
背景：自体脂肪移植是一种常用的修复软组织缺损的策略，在过去的5年中，其使用率增加了约40%。然而，高重吸收率（平均50%）往往导致不满意的结果。目前旨在增加移植脂肪血液供应的方法几乎没有临床支持。在这里，我们发现，丹参可以提高脂肪移植物的存活率，通过促进脂肪来源的干细胞的成脂分化的过氧化物酶体增殖物激活受体γ（PPAR γ）和CCAAT-增强子结合蛋白α（C/EBP α）signaling.Methods：脂肪组织收获两名妇女的大腿。通过流式细胞术（CD 29、CD 90和CD 105）对脂肪来源的干细胞进行表征。将样品（2 × 10（4）个细胞/升）与或不与S.丹参注射液（0.001、0.005、0.01、0.05、0.1、0.5、1和5 g/L）。油红O染色、甘油三酯含量和成脂基因表达结果：0.5g/L组甘油三酯含量显著高于对照组（P < 0.01），且随着浓度的增加，甘油三酯含量逐渐升高，随着浓度的增加，甘油三酯含量逐渐升高，随着浓度的增加，甘油三酯含量逐渐升高，随着浓度的增加，甘油三酯含量逐渐升高，随着浓度的增加，甘油三酯含量逐渐升高（0.231 +/- 0.010，76.90%相对于对照，p < 0.001，第9天; 0.303 +/- 0.010，91.28%相对于对照，p < 0.001，第10天; 0.361 +/- 0.008，86.65%相对于对照，p < 0.001，第11天）。与对照组相比，0.5-g/L组的PPAR γ和C/EBP α的表达水平均显著增加（0.0097 +/- 0.0015，48.1% vs对照组，p < 0.05对于PPAR γ; 0.0423 +/- 0.003，112% vs对照组，p < 0.001对于C/EBP α）。S.丹参注射液对脂肪干细胞体外成脂有促进作用。这种治疗对提高脂肪移植物存活率的作用需要更多的体内研究。
Background:Autologous fat grafting is a commonly used strategy to repair soft-tissue defects that has shown an approximately 40 percent increase in use in the past 5 years. However, the high reabsorption rates (average, 50 percent) often result in an unsatisfactory outcome. Current approaches aimed at increasing the blood supply of grafted fat have little clinical support. Here, we found that Salvia miltiorrhiza could improve fat graft survival by promoting adipogenic differentiation of adipose-derived stem cells by means of peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT-enhancer binding protein alpha (C/EBP alpha) signaling.Methods:Adipose tissue was harvested from the thighs of two women. Adipose-derived stem cells were characterized by flow cytometry (CD29, CD90, and CD105). The samples (2 x 10(4) cells/liter) were incubated with or without S. miltiorrhiza injection (0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, and 5 g/liter) during adipogenic differentiation. Oil Red O staining, triglyceride content, and adipogenic gene expression (PPAR gamma and C/EBP alpha) were performed to detect adipogenic differentiation.Results:The triglyceride content in the 0.5-g/liter group was increased significantly compared with that in control groups (0.231 +/- 0.010, 76.90 percent versus control, p < 0.001, day 9; 0.303 +/- 0.010, 91.28 percent versus control, p < 0.001, day 10; 0.361 +/- 0.008, 86.65 percent versus control, p < 0.001, day 11). The expression levels of PPAR gamma and C/EBP alpha in the 0.5-g/liter group were both increased significantly compared with those in control groups (0.0097 +/- 0.0015, 48.1 percent versus control, p < 0.05 for PPAR gamma; 0.0423 +/- 0.003, 112 percent versus control, p < 0.001 for C/EBP alpha).Conclusions:S. miltiorrhiza injection has a positive effect on adipogenesis of adipose-derived stem cells in vitro. The effect of this treatment on improving fat graft survival needs more in vivo research.