Quantitative profiling analysis of organic acids in complex mixtures.
Quantitative profiling analysis of organic acids in complex mixtures.
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复杂混合物中有机酸的定量分析。
DOI：
10.1002/bms.1200080915
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Issachar,D
中科院分区：
文献类型：
--
作者：
Sweeley,CC;Vrbanac,J;Pinkston,D;Issachar,D
同时检测和定量测定生理液体或组织中大量代谢物，并随后将这些数据与参考值进行比较，是通常称为“代谢谱”分析的过程。在填充柱或毛细管柱上通过气液色谱法生成的图谱很容易通过质谱法进行分析，利用反向库搜索程序来识别单个组分。本文将重点介绍血浆中有机酸的预纯化程序、石英毛细管在代谢谱分析中的应用以及该方法对痕量组分的灵敏度。
The simultaneous detection and quantitative determination of a very large number of metabolites in physiological fluids or tissues, and the subsequent comparison of these data with reference values, is a process which has commonly been called “metabolic profiling” analysis. Profiles generated by gas‐liquid chromatography on packed or capillary columns are readily analyzed by mass spectrometry, utilizing reverse library search procedures to identify individual components. Emphasis will be given here to procedures for the pre‐purification of organic acids from plasma, to the use of quartz capillaries for metabolic profiling analysis, and to the sensitivity of this method for trace components.