Origin and distribution of AT-rich repetitive DNA families in Triatoma infestans (Heteroptera)
Origin and distribution of AT-rich repetitive DNA families in Triatoma infestans (Heteroptera)
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DOI：
10.1016/j.meegid.2014.01.035
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发表时间：
2014-04-01
影响因子：
3.2
通讯作者：
Laforga Vanzela, Andre Luis
中科院分区：
文献类型：
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作者：
Bardella, Vanessa Bellini;da Rosa, Joao Aristeu;Laforga Vanzela, Andre Luis
致病锥虫是克氏锥虫最重要的传播媒介之一，是一个非常有趣的模式虫，因为它在异染色质的数量和分布上表现出很大的种群间差异。这种多态性涉及三个大的对几乎所有的常染色体对，包括性染色体。了解T. infestans中，我们分离的AT-丰富的satDNA部分，这种昆虫使用重新结合动力学（C（0）t），然后克隆，测序和FISH。染色体定位后，用抗5-甲基胞嘧啶、抗H4 K5 ac和抗H3 K9 me 2抗体进行免疫标记以确定异染色质的功能特征。这些结果使我们能够重组T.根据致病菌的重复DNA家族的分布，使用7个不同的标记。我们发现两个长度分别为79和33 bp的序列与转座因子序列有很强的相关性，这表明这两个家族的satDNA可能起源于Polintons。这些结果还使我们能够鉴定出至少四种与T.害虫这些数据在新的研究中应该是非常有用的，包括那些检查这种非常重要的昆虫物种的细胞基因组和种群方面。(C)2014爱思唯尔有限公司版权所有。
Triatoma infestans, one of the most important vectors of Trypanosoma cruzi, is very interesting model, because it shows large interpopulation variation in the amount and distribution of heterochromatin. This polymorphism involved the three large pairs up to almost all autosomal pairs, including the sex chromosomes. To understand the dynamics of heterochromatin variation in T. infestans, we isolated the AT-rich satDNA portion of this insect using reassociation kinetics (C(0)t), followed by cloning, sequencing and FISH. After chromosome localization, immunolabeling with anti-5-methylcytosine, anti-H4K5ac and anti-H3K9me2 antibodies was performed to determine the functional characteristics of heterochromatin. The results allowed us to reorganize the karyotype of T. infestans in accordance with the distribution of the families of repetitive DNA using seven different markers. We found that two arrays with lengths of 79 and 33 bp have a strong relationship with transposable element sequences, suggesting that these two families of satDNA probably originated from Polintons. The results also allowed us to identify at least four chromosome rearrangements involved in the amplification/dispersion of AT-rich satDNA of T. infestans. These data should be very useful in new studies including those examining the cytogenomic and population aspects of this very important species of insect. (C) 2014 Elsevier B.V. All rights reserved.