Estrogenic activity of tissue conditioners in vitro
Estrogenic activity of tissue conditioners in vitro
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DOI：
10.1016/s0109-5641(02)00064-7
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发表时间：
2003-06-01
期刊：
影响因子：
5
通讯作者：
Nakamura, M
中科院分区：
文献类型：
--
作者：
Hashimoto, Y;Kawaguchi, M;Nakamura, M
目标。为了评估组织调理剂中使用的增塑剂和四种商业组织调理剂的雌激素活性，我们进行了体外试验。七种增塑剂和两种代谢物被稀释至浓度范围为10(-9)至10(-4) M。四种商业组织调理剂也被稀释至浓度范围为2 X 10(-8)至2 X 10(-3) g/ml。使用 MCF-7 细胞通过电子筛选测试测试雌激素活性。当雌激素存在时，细胞就会增殖。不是直接计数细胞或细胞核，而是使用磺基罗丹明 B 测定通过测量总蛋白含量来评估细胞数量。通过高效液相色谱法检查了四种市售组织调理剂的液体成分。结果。邻苯二甲酸正丁基苄酯、邻苯二甲酸二丁酯、邻苯二甲酸正丁基乙醇酸正丁酯、邻苯二甲酸二-2-乙基己酯和水杨酸苄酯显着增加MCF-7细胞的增殖。其余两种增塑剂，己二酸二-2-乙基己酯和苯甲酸苄酯，以及邻苯二甲酸二丁酯和邻苯二甲酸二-2-乙基己酯的两种代谢物，分别是邻苯二甲酸单丁酯和邻苯二甲酸单-2-乙基己酯，在测试浓度下不会增加MCF-7细胞的增殖。四种商业组织调理剂，Coe Comfort (CC)、Tissue Conditioner (TC)、Hydro Cast (HQ 和 Denture Soft (DS) II) 显着增加了 MCF-7 细胞的增殖。HPLC 数据显示商业产品含有增塑剂：CC 中的苯甲酸苄酯和邻苯二甲酸二丁酯、TC 中的邻苯二甲酸二丁酯、HC 中的邻苯二甲酸正丁酯和 DS H 中的邻苯二甲酰正丁基乙醇酸。苯甲酸苄酯和己二酸二-2-乙基己酯，测试的四种商业组织调理剂也显示出雌激素活性，并且HC显示出特别强的雌激素活性（C）2003年牙科材料学会出版。保留所有权利。
Objectives. In order to assess the estrogenic activities of plasticizers used in tissue conditioners and four commercial tissue conditioners, we carried out in vitro tests.Methods. Seven plasticizers and two metabolites were diluted to concentrations ranging from 10(-9) to 10(-4) M. Four commercial tissue conditioners were also diluted to concentrations ranging from 2 X 10(-8) to 2 X 10(-3) g/ml. Estrogenic activities were tested by the E-screen test using MCF-7 cells. When estrogen is present, the cells proliferate. Instead of counting the cells or nuclei directly, cell numbers were assessed by measurement of the total protein content using the sulforhodamine B assay. The liquid compositions of the four commercial tissue conditioners were examined by high-performance liquid chromatography.Results. n-Butyl benzyl phthalate, dibutyl phthalate, n-butyl phthalyl n-butyl glycolate, di-2-ethylhexyl phthalate and benzyl salicylate significantly increased proliferation of MCF-7 cells. The remaining two plasticizers, di-2-ethylhexyl adipate and benzyl benzoate, as well as two metabolites of dibutyl phthalate and di-2-ethylhexyl phthalate, i.e. monobutyl phthalate and mono-2-ethylhexyl phthalate, respectively, did not increase proliferation of MCF-7 cells at the concentrations tested. Four commercial tissue conditioners, Coe comfort (CC), Tissue Conditioner (TC), Hydro Cast (HQ and Denture Soft (DS) II, significantly increased proliferation of MCF-7 cells. HPLC data revealed the commercial products contained plasticizers: benzyl benzoate and dibutyl phthalate in CC, dibutyl phthalate in TC, n-butyl benzyl phthalate in HC and n-butyl phthalyl n-butyl glycolate in DS H.Significance. Except for benzyl benzoate and di-2-ethylhexyl adipate, the plasticizers tested showed estrogenic activity. The four commercial tissue conditioners tested also showed estrogenic activity, and HC showed especially strong estrogenicity. (C) 2003 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.