Minimum set of cytochromes P450 for reconstituting the biosynthesis of camalexin, a major Arabidopsis antibiotic.
Minimum set of cytochromes P450 for reconstituting the biosynthesis of camalexin, a major Arabidopsis antibiotic.
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最低限度的细胞色素P450用于重建Camalexin（一种主要的拟南芥抗生素）的生物合成。
DOI：
10.1002/anie.201307454
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发表时间：
2013-12-16
期刊：
影响因子：
--
通讯作者：
Sattely ES
中科院分区：
文献类型：
--
作者：
Klein AP;Anarat-Cappillino G;Sattely ES
通过体外生物化学表征，发现了拟南芥的模式抗生素camalexin生物合成中的关键缺失步骤。Trp和Cys衍生片段通过C-S键形成的偶联由不寻常的细胞色素P450 CYP 71 A13促进。仅使用三个P450实现了从Trp和Cys的Camalexin生物合成的首次体外重建。
The discovery of the key missing step in the biosynthesis of camalexin, a model antibiotic from Arabidopsis, was uncovered through in vitro biochemical characterization. The coupling of Trp- and Cys-derived fragments via C–S bond formation is promoted by an unusual cytochrome P450 CYP71A13. The first in vitro reconstitution of camalexin biosynthesis from Trp and Cys was achieved using just three P450s.