Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem
Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem
复制标题
铁皮石斛茎多糖不同提取方法比较
DOI：
10.1016/j.carbpol.2018.06.073
复制
发表时间：
2018-10-15
影响因子：
11.2
通讯作者：
Huang, Song
中科院分区：
文献类型：
--
作者：
He, Lian;Yan, Xiaoteng;Huang, Song
筛选铁皮石斛茎中多糖的最佳提取工艺。首先，采用热水浸提法、冷榨法、冻融冷榨法、超声波辅助热水浸提法、微波辅助热水浸提法和酶辅助热水浸提法，在各自的最佳工艺条件下提取DOP。在相同条件下，比较不同提取方法所得多糖的提取率、结构及抗氧化活性。结果表明，UHWE和FTCP的提取率高于其他提取方法。此外，DOPCP和DOPFTCP具有比其他多糖样品更高的分子量。更重要的是，DOPFTCP具有最高的抗氧化活性。结果表明，FTCP法提取DOP的得率高，分子链完整，抗氧化活性最好，是提取DOP的最佳方法。
The purpose of this study was to screen the optimum extraction of polysaccharides (DOP) from Dendrobium officinale stem. Firstly, different methods, including hot water extraction (HWE), cold-pressing (CP), freeze-thawing cold-pressing (FTCP), ultrasonic-assisted hot water extraction (UHWE), microwave-assisted hot water extraction (MHWE) and enzyme-assisted hot water extraction (EHWE), were employed to extract DOP under their respective best parameters. Then, the extraction yield, structure and antioxidant activity of the polysaccharides from different extraction methods were compared under the same condition. The data implied that UHWE and FTCP possessed higher extraction yield than the other extraction methods. Besides, DOPCP and DOPFTCP had higher molecular weight than the other polysaccharide samples. More importantly, DOPFTCP had the highest antioxidant activity. Overall, DOPFTCP exhibit high extraction yield, well-preserved molecular chains and best antioxidant activity, all these indicated FTCP was the most suitable method to extract DOP.