Normalized transillumination of fluorescent proteins in small animals
Normalized transillumination of fluorescent proteins in small animals
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DOI：
10.2310/7290.2006.00018
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发表时间：
2006-07-01
影响因子：
2.8
通讯作者：
Ntziachristos, Vasilis
中科院分区：
文献类型：
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作者：
Zacharakis, Giannis;Shih, Helen;Ntziachristos, Vasilis
我们研究了标准化透照方法在可见光、幻影和活小鼠中成像荧光蛋白的能力。我们展示了显着的成像改进落射照明成像，如今天普遍应用。特别重要的是，与落射照明成像相比，由于最大限度地减少了自发荧光的影响并增强了深度灵敏度，因此实现了对比度的显着改善。虽然归一化透照不匹配的准确性和定量能力的断层成像，但它仍然获得了关键的优势，其他平面光学成像方法，同时保持实现简单性。由于实现了简单性和准确性之间的平衡，归一化透照方法可以作为一个重要的替代分子成像方法。
We investigated the capacity of normalized transillumination methods in imaging fluorescent proteins in visible light, in phantoms and in live mice. We demonstrate significant imaging improvements over epi-illumination imaging, as commonly applied today. Of particular importance is the significant betterment in contrast achieved, due to minimization of the influence of autofluorescence and the enhancement of depth sensitivity compared to epi-illumination imaging. Although normalized transillumination does not match the accuracy and quantification capacity of tomographic imaging, it nevertheless attains key advantages over other planar optical imaging methods while preserving implementation simplicity. Due to the balance achieved between simplicity and accuracy, normalized transillumination approaches could serve as an important alternative molecular imaging method.