Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo
Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo
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DOI：
10.1194/jlr.m500420-jlr200
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发表时间：
2006-05-01
影响因子：
6.5
通讯作者：
Chung, Jin Ho
中科院分区：
文献类型：
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作者：
Kim, Hyeon Ho;Cho, Soyun;Chung, Jin Ho
皮肤老化可归因于光老化（外在）和时间老化（内在）。光老化和内在老化分别是由于人体皮肤反复暴露于紫外线（UV）照射和时间的流逝而引起的损伤。在我们之前的报道中，二十碳五烯酸（EPA）被发现可以抑制紫外线诱导的人真皮成纤维细胞中基质金属蛋白酶-1 （MMP-1）的表达。因此，我们研究了EPA对紫外线诱导的皮肤损伤和内在衰老的影响，分别将EPA局部应用于年轻和年老的皮肤。通过免疫组化分析和Western blotting，我们发现局部应用EPA可减少紫外线诱导的表皮增厚，抑制紫外线诱导的胶原减少。还发现EPA通过抑制与uv诱导的激活蛋白1激活密切相关的c-Jun磷酸化，以及抑制JNK和p38的激活，减弱了uv诱导的MMP-1和MMP-9的表达。EPA还能抑制紫外线诱导的环氧合酶-2 （COX-2）的表达，但不改变COX-1的表达。此外，EPA通过增加转化蛋白生长因子- β的表达，增加了衰老皮肤中胶原蛋白和弹性纤维（tropoelastin和fibrin -1）的表达。总之，这些结果表明，局部EPA有潜力作为一种抗皮肤老化剂。
Skin aging can be attributed to photoaging (extrinsic) and chronological (intrinsic) aging. Photoaging and intrinsic aging are induced by damage to human skin attributable to repeated exposure to ultraviolet (UV) irradiation and to the passage of time, respectively. In our previous report, eicosapentaenoic acid (EPA) was found to inhibit UV-induced matrix metalloproteinase-1 (MMP-1) expression in human dermal fibroblasts. Therefore, we investigated the effects of EPA on UV-induced skin damage and intrinsic aging by applying EPA topically to young and aged human skin, respectively. By immunohistochemical analysis and Western blotting, we found that topical application of EPA reduced UV-induced epidermal thickening and inhibited collagen decrease induced by UV light. It was also found that EPA attenuated UV-induced MMP-1 and MMP-9 expression by inhibiting UV-induced c-Jun phosphorylation, which is closely related to UV-induced activator protein-1 activation, and by inhibiting JNK and p38 activation. EPA also inhibited UV-induced cyclooxygenase-2 (COX-2) expression without altering COX-1 expression. Moreover, it was found that EPA increased collagen and elastic fibers (tropoelastin and fibrillin-1) expression by increasing transformin growth factor-beta expression in aged human skin. Together, these results demonstrate that topical EPA has potential as an anti-skin-aging agent.