De novo assembly and transcriptome characterization: novel insights into the natural resistance mechanisms of Microtus fortis against Schistosoma japonicum.
De novo assembly and transcriptome characterization: novel insights into the natural resistance mechanisms of Microtus fortis against Schistosoma japonicum.
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从头组装和转录组表征：对东方田鼠对日本血吸虫天然抗性机制的新见解
DOI：
10.1186/1471-2164-15-417
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发表时间：
2014-06-02
期刊：
影响因子：
4.4
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Hu Y;Xu Y;Lu W;Yuan Z;Quan H;Shen Y;Cao J
背景：东方田鼠是日本血吸虫的非允许宿主。它对血吸虫有天然的抵抗力，但确切的抵抗力机制尚不清楚。福尔蒂斯分枝杆菌遗传信息的缺乏限制了可用的免疫学方法的使用。因此，需要基于高通量测序技术的研究来获得关于日本血吸虫抗性机制的信息。结果：利用Illumina单端技术，东方分枝杆菌转录组的从头组装产生了67,751个非基因组，平均长度为868个核苷酸。用RNA-seq定量分析方法对日本血吸虫感染前后的东方分支杆菌进行了比较。差异表达基因最多出现在感染后2周，下调基因最多出现在感染后3周。同时，肝组织病理改变在2周时最为明显。与DEGS相关的基因本体论术语和途径表明，上调的转录本参与新陈代谢、免疫和炎症反应。实时定量聚合酶链式反应分析结果显示基因表达模式与RNA-seq结果一致。结论：东亚支原体感染日本血吸虫后迅速产生防御反应，在感染后第2周急剧增加，感染后3周逐渐减弱。获得的东方分枝杆菌转录组和DEGS谱数据表明，自然和获得性免疫反应在东方分枝杆菌对日本血吸虫的免疫中起着重要作用。这些发现为了解东方分枝杆菌对血吸虫的天然抗性机制提供了更好的理解。
Background:Microtus fortis is a non-permissive host of Schistosoma japonicum. It has natural resistance against schistosomes, although the precise resistance mechanisms remain unclear. The paucity of genetic information for M. fortis limits the use of available immunological methods. Thus, studies based on high-throughput sequencing technologies are required to obtain information about resistance mechanisms against S. japonicum.Results:Using Illumina single-end technology, a de novo assembly of the M. fortis transcriptome produced 67,751 unigenes with an average length of 868 nucleotides. Comparisons were made between M. fortis before and after infection with S. japonicum using RNA-seq quantification analysis. The highest number of differentially expressed genes (DEGs) occurred two weeks after infection, and the highest number of down-regulated DEGs occurred three weeks after infection. Simultaneously, the strongest pathological changes in the liver were observed at week two. Gene ontology terms and pathways related to the DEGs revealed that up-regulated transcripts were involved in metabolism, immunity and inflammatory responses. Quantitative real-time PCR analysis showed that patterns of gene expression were consistent with RNA-seq results.Conclusions:After infection with S. japonicum, a defensive reaction in M. fortis commenced rapidly, increasing dramatically in the second week, and gradually decreasing three weeks after infection. The obtained M. fortis transcriptome and DEGs profile data demonstrated that natural and adaptive immune responses, play an important role in M. fortis immunity to S. japonicum. These findings provide a better understanding of the natural resistance mechanisms of M. fortis against schistosomes.
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