Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis
Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis
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DOI：
10.1016/j.lfs.2009.08.003
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发表时间：
2009-09-23
期刊：
影响因子：
6.1
通讯作者：
Chiou, Tzyy-Wen
中科院分区：
文献类型：
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作者：
Chang, Yao-Jen;Liu, Jen-Wea;Chiou, Tzyy-Wen
目的：为探讨人骨髓间充质干细胞（human bone marrow mesenchymal stem cells，hBMMSCs）修复肝损伤的可行性及其机制，我们采用四氯化碳（CCl4）诱导的大鼠肝损伤模型，研究了hBMMSCs修复肝损伤（包括肝纤维化）的潜力。将GFP标记的hBMMSCs经门静脉注射入大鼠体内。主要发现：移植后第1天，GFP标记的hBMMSCs出现在肝小叶周围、肝血管周围和肝叶边缘。生化和组织病理学分析表明，移植组的肝损伤恢复显着增加。此外，移植的hBMMSCs表达基质金属蛋白酶（MMP），肝纤维化明显减轻。肝纤维化程度的减少与肝切片中hBMMSCs的数量密切相关。意义：我们的数据表明，hBMMSCs可能有助于慢性肝损伤的恢复，并可能减少肝纤维化。因此，hBMMSCs是治疗肝硬化的潜在选择。(C)2009 Elsevier Inc. All rights reserved.
Aims: To investigate the feasibility and mechanism of liver damage repair using human bone marrow mesenchymal stem cells (hBMMSCs), we investigated the potential for hBMMSCs in recovery from liver damage, including fibrotic liver repair, using the CCl4-induced model for liver damage in the rat.Main methods: Rats were injected with 0.5 ml/kg CCl4 to induce liver damage and progressive liver fibrosis. hBMMSCs labeled with GFP were injected into the rats through the portal vein.Key findings: After one day of transplantation, GFP-labeled cells were found around the liver lobules, the hepatic blood vessels, and the edge of the liver lobes. Biochemical and histopathological analyses showed significantly increased recovery from liver damage in the transplanted group. In addition, transplanted hBMMSCs express matrix metalloproteinases (MMP), and liver fibrosis was significantly decreased. The degree of fibrosis reduction paralleled the number of hBMMSCs observed in liver sections.Significance: Our data suggest that hBMMSCs may facilitate recovery from chronic liver damage and may decrease liver fibrosis. Therefore, hBMMSCs are a potential option for treatment of liver cirrhosis. (C) 2009 Elsevier Inc. All rights reserved.