Regulation of lubricin/superficial zone protein by Wnt signalling in bovine synoviocytes.
Regulation of lubricin/superficial zone protein by Wnt signalling in bovine synoviocytes.
复制标题
牛滑膜细胞中 Wnt 信号传导对润滑素/表面区蛋白的调节。
DOI：
10.1002/term.1808
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发表时间：
2016
影响因子：
3.3
通讯作者：
HariReddi,A
中科院分区：
文献类型：
--
作者：
Inui,Atsuyuki;Iwakura,Takashi;HariReddi,A
润滑素，同源于表浅区蛋白（SZP），在关节软骨中起边界润滑剂的作用，并在维持关节功能和稳态中起重要作用。Wnt信号传导在关节发育（包括滑膜关节形成）中起关键作用，并且在关节炎的滑膜和关节软骨中表达几种Wnt蛋白。本研究的目的是确定Wnt信号传导对滑膜细胞中SZP积累的作用。将来自牛膝关节的分离的滑膜细胞与Wnt蛋白（Wnt-3a和Wnt-5a）和Wnt-β-连环蛋白途径或Wnt-Ca 2+途径的拮抗剂或激动剂一起在无血清化学成分确定的培养基中培养。通过酶联免疫吸附试验测定培养基中的SZP蓄积。Wnt-3a通过Wnt-β-catenin-依赖性途径抑制SZP积累。相反，Wnt-5a通过β-连环蛋白非依赖性途径刺激SZP积累。目前的调查提供了新的见解的作用，Wnt信号通路的SZP积累在滑膜细胞和正常关节的稳态中的作用。版权所有© 2013约翰威利父子有限公司.
Lubricin, homologous to superficial zone protein (SZP), functions as a boundary lubricant in articular cartilage and plays an essential role in the maintenance of joint function and homeostasis. Wnt signalling plays a key role in joint development, including synovial joint formation, and several Wnt proteins are expressed in the synovium and articular cartilage in arthritis. The aim of this study was to determine the role of Wnt signalling on SZP accumulation in synoviocytes. Isolated synoviocytes from bovine knee joints were cultured with Wnt proteins (Wnt‐3a and Wnt‐5a) and antagonists or agonists of the Wnt–β‐catenin pathway or Wnt–Ca2+pathway in serum‐free chemically defined medium. SZP accumulation in the culture medium was determined by enzyme‐linked immunosorbent assay. Wnt‐3a suppressed SZP accumulation via a Wnt–β‐catenin‐dependent pathway. In contrast, Wnt‐5a stimulated SZP accumulation via aβ‐catenin independent pathway. The present investigation provides novel insights into the role of the Wnt signalling pathways in SZP accumulation in synoviocytes and their roles in the homeostasis of normal joints. Copyright © 2013 John Wiley & Sons, Ltd.